{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pezzotti G"],"funding":["NIDCR NIH HHS"],"pagination":["6694"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10095091"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(7)"],"pubmed_abstract":["This study probed in vitro the mechanisms of competition/coexistence between <i>Streptococcus sanguinis</i> (known for being correlated with health in the oral cavity) and <i>Streptococcus mutans</i> (responsible for aciduric oral environment and formation of caries) by means of quantitative Raman spectroscopy and imaging. In situ Raman assessments of live bacterial culture/coculture focusing on biofilm exopolysaccharides supported the hypothesis that both species engaged in antagonistic interactions. Experiments of simultaneous colonization always resulted in coexistence, but they also revealed fundamental alterations of the biofilm with respect to their water-insoluble glucan structure. Raman spectra (collected at fixed time but different bacterial ratios) showed clear changes in chemica"],"journal":["International journal of molecular sciences"],"pubmed_title":["In Situ Raman Analysis of Biofilm Exopolysaccharides Formed in <i>Streptococcus mutans</i> and <i>Streptococcus sanguinis</i> Commensal Cultures."],"pmcid":["PMC10095091"],"funding_grant_id":["R01 DE029709","R21 DE028715","R15 DE027851"],"pubmed_authors":["Ouhara K","Kimura S","Togo A","Yamamoto T","Shiba H","Kawai T","Pezzotti G","Ofuji S","Iwata T","Ohgitani E","Marin E","Kanamura N","Mazda O","Aoki T","Imamura H","Zhu W","Adachi T"],"additional_accession":[]},"is_claimable":false,"name":"In Situ Raman Analysis of Biofilm Exopolysaccharides Formed in <i>Streptococcus mutans</i> and <i>Streptococcus sanguinis</i> Commensal Cultures.","description":"This study probed in vitro the mechanisms of competition/coexistence between <i>Streptococcus sanguinis</i> (known for being correlated with health in the oral cavity) and <i>Streptococcus mutans</i> (responsible for aciduric oral environment and formation of caries) by means of quantitative Raman spectroscopy and imaging. In situ Raman assessments of live bacterial culture/coculture focusing on biofilm exopolysaccharides supported the hypothesis that both species engaged in antagonistic interactions. Experiments of simultaneous colonization always resulted in coexistence, but they also revealed fundamental alterations of the biofilm with respect to their water-insoluble glucan structure. Raman spectra (collected at fixed time but different bacterial ratios) showed clear changes in chemica","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2025-04-18T15:19:54.678Z","creation":"2025-04-07T01:54:56.276Z"},"accession":"S-EPMC10095091","cross_references":{"pubmed":["37047667"],"doi":["10.3390/ijms24076694"]}}