biostudies-literatureBatisti Biffignandi GMinistero della SaluteMinistero dell’Istruzione, dell’Università e della Ricercae0011210https://www.ebi.ac.uk/biostudies/studies/S-EPMC10096192biostudies-literatureUnknown17(4)<h4>Background</h4>Cystic echinococcosis (CE), caused by Echinococcus granulosus sensu lato, is a neglected zoonosis. Its diagnosis relies on imaging, supported by serology, while only imaging is useful for staging and follow-up. Since diagnostic tools and expertise are not widely available, new accurate and easily implementable assays for the diagnosis and follow-up of CE are highly needed.<h4>Methodology/principal findings</h4>We aimed to identify new E. granulosus antigens through a bioinformatics selection applied to the parasite genome, followed by peptide microarray screening and validation in ELISA, using independent panels of sera from patients with hepatic CE and clinically relevant controls. From 950 proteins selected in silico, 2,379 peptides were evaluated by microarray for IgGPLoS neglected tropical diseasesAntigen discovery by bioinformatics analysis and peptide microarray for the diagnosis of cystic echinococcosis.PMC10096192Linea 1-Ricerca Corrente to INMIDipartimenti di Eccellenza ProgramRicerca Corrente L3P2 to Sacro Cuore Don Calabria HospitalGR-2016-02361497Vola ABrunetti ETamarozzi FNajafi-Fard STeggi ABatisti Biffignandi GSassera DPetrone LGoletti DGomez Morales MAfalseAntigen discovery by bioinformatics analysis and peptide microarray for the diagnosis of cystic echinococcosis.<h4>Background</h4>Cystic echinococcosis (CE), caused by Echinococcus granulosus sensu lato, is a neglected zoonosis. Its diagnosis relies on imaging, supported by serology, while only imaging is useful for staging and follow-up. Since diagnostic tools and expertise are not widely available, new accurate and easily implementable assays for the diagnosis and follow-up of CE are highly needed.<h4>Methodology/principal findings</h4>We aimed to identify new E. granulosus antigens through a bioinformatics selection applied to the parasite genome, followed by peptide microarray screening and validation in ELISA, using independent panels of sera from patients with hepatic CE and clinically relevant controls. From 950 proteins selected in silico, 2,379 peptides were evaluated by microarray for IgG2023-01-01T00:00:00Z2023 Apr2025-04-05T11:29:26.734Z2025-02-18T23:40:45.382ZS-EPMC100961923704348910.1371/journal.pntd.0011210