biostudies-literatureUnknown61(51)Couturier CHerein, we describe the myxobacterial natural product Corramycin isolated from Corallococcus coralloides. The linear peptide structure contains an unprecedented (2R,3S)-γ-N-methyl-β-hydroxy-histidine moiety. Corramycin exhibits anti-Gram-negative activity against Escherichia coli (E. coli) and is taken up via two transporter systems, SbmA and YejABEF. Furthermore, the Corramycin biosynthetic gene cluster (BGC) was identified and a biosynthesis model was proposed involving a 12-modular non-ribosomal peptide synthetase/polyketide synthase. Bioinformatic analysis of the BGC combined with the development of a total synthesis route allowed for the elucidation of the molecule's absolute configuration. Importantly, intravenous administration of 20 mg kg^-1 of Corramycin in an E. coli moAngewandte Chemie (International ed. in English)e202210747https://www.ebi.ac.uk/biostudies/studies/S-EPMC10099666biostudies-literatureStructure Elucidation, Total Synthesis, Antibacterial In Vivo Efficacy and Biosynthesis Proposal of Myxobacterial Corramycin.PMC10099666Vermat TBauer ACazals VSchummer DMuller RTaillier TDeckarm SCouturier CDubarry NLeroi-Geissler CSizun PRey AToti LHaag Richter SRenard SKurz MPoverlein CHarmrolfs Kvon Tesmar ASilve SStump HWink JGroß SMourez MBacque EFievet ALessoud EFraisse LVersluys SHoffmann JZaburannyi NHoffmann MPrasad Awal RfalseStructure Elucidation, Total Synthesis, Antibacterial In Vivo Efficacy and Biosynthesis Proposal of Myxobacterial Corramycin.Herein, we describe the myxobacterial natural product Corramycin isolated from Corallococcus coralloides. The linear peptide structure contains an unprecedented (2R,3S)-γ-N-methyl-β-hydroxy-histidine moiety. Corramycin exhibits anti-Gram-negative activity against Escherichia coli (E. coli) and is taken up via two transporter systems, SbmA and YejABEF. Furthermore, the Corramycin biosynthetic gene cluster (BGC) was identified and a biosynthesis model was proposed involving a 12-modular non-ribosomal peptide synthetase/polyketide synthase. Bioinformatic analysis of the BGC combined with the development of a total synthesis route allowed for the elucidation of the molecule's absolute configuration. Importantly, intravenous administration of 20 mg kg^-1 of Corramycin in an E. coli mo2022-01-01T00:00:00Z2022 Dec2025-04-05T12:13:16.062Z2025-04-05T12:13:16.062ZS-EPMC100996663619775510.1002/anie.202210747