{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["46(2)"],"submitter":["Vidal-Meireles A"],"funding":["Alexander von Humboldt-Stiftung","Magyar Tudományos Akadémia","Nemzeti Kutatási Fejlesztési és Innovációs Hivatal"],"pubmed_abstract":["PSBO is essential for the assembly of the oxygen-evolving complex in plants and green algae. Despite its importance, we lack essential information on its lifetime and how it depends on the environmental conditions. We have generated nitrate-inducible PSBO amiRNA lines in the green alga Chlamydomonas reinhardtii. Transgenic strains grew normally under non-inducing conditions, and their photosynthetic performance was comparable to the control strain. Upon induction of the PSBO amiRNA constructs, cell division halted. In acetate-containing medium, cellular PSBO protein levels decreased by 60% within 24 h in the dark, by 75% in moderate light, and in high light, the protein completely degraded. Consequently, the photosynthetic apparatus became strongly damaged, probably due to 'donor-side-indu"],"journal":["Plant, cell & environment"],"pagination":["422-439"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10100022"],"repository":["biostudies-literature"],"pubmed_title":["The lifetime of the oxygen-evolving complex subunit PSBO depends on light intensity and carbon availability in Chlamydomonas."],"pmcid":["PMC10100022"],"pubmed_authors":["Neupert J","Szeles E","Vidal-Meireles A","Kuntam S","Toth D","Bock R","Toth SZ"],"additional_accession":[]},"is_claimable":false,"name":"The lifetime of the oxygen-evolving complex subunit PSBO depends on light intensity and carbon availability in Chlamydomonas.","description":"PSBO is essential for the assembly of the oxygen-evolving complex in plants and green algae. Despite its importance, we lack essential information on its lifetime and how it depends on the environmental conditions. We have generated nitrate-inducible PSBO amiRNA lines in the green alga Chlamydomonas reinhardtii. Transgenic strains grew normally under non-inducing conditions, and their photosynthetic performance was comparable to the control strain. Upon induction of the PSBO amiRNA constructs, cell division halted. In acetate-containing medium, cellular PSBO protein levels decreased by 60% within 24 h in the dark, by 75% in moderate light, and in high light, the protein completely degraded. Consequently, the photosynthetic apparatus became strongly damaged, probably due to 'donor-side-indu","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-04T14:23:40.145Z","creation":"2024-12-04T12:25:12.835Z"},"accession":"S-EPMC10100022","cross_references":{"pubmed":["36320098"],"doi":["10.1111/pce.14481"]}}