biostudies-literatureElfers KNIH/SPARCe0282732https://www.ebi.ac.uk/biostudies/studies/S-EPMC10101454biostudies-literatureUnknown18(4)It was suggested that intestinal mucosal secretion is enhanced during muscle relaxation and contraction. Mechanisms of mechanically induced secretion have been studied in rodent species. We used voltage clamp Ussing technique to investigate, in human and porcine colonic tissue, secretion evoked by serosal (Pser) or mucosal (Pmuc) pressure application (2-60 mmHg) to induce distension into the mucosal or serosal compartment, respectively. In both species, Pser or Pmuc caused secretion due to Cl- and, in human colon, also HCO3- fluxes. In the human colon, responses were larger in proximal than distal regions. In porcine colon, Pmuc evoked larger responses than Pser whereas the opposite was the case in human colon. In both species, piroxicam revealed a strong prostaglandin (PG) dependent compoPloS oneDistension evoked mucosal secretion in human and porcine colon in vitro.PMC101014541OT2OD0248999Michel KZeller FSchauffele SDemir IEElfers KHoppe SMazzuoli-Weber GSchemann MfalseDistension evoked mucosal secretion in human and porcine colon in vitro.It was suggested that intestinal mucosal secretion is enhanced during muscle relaxation and contraction. Mechanisms of mechanically induced secretion have been studied in rodent species. We used voltage clamp Ussing technique to investigate, in human and porcine colonic tissue, secretion evoked by serosal (Pser) or mucosal (Pmuc) pressure application (2-60 mmHg) to induce distension into the mucosal or serosal compartment, respectively. In both species, Pser or Pmuc caused secretion due to Cl- and, in human colon, also HCO3- fluxes. In the human colon, responses were larger in proximal than distal regions. In porcine colon, Pmuc evoked larger responses than Pser whereas the opposite was the case in human colon. In both species, piroxicam revealed a strong prostaglandin (PG) dependent compo2023-01-01T00:00:00Z20232026-04-08T12:27:59.679Z2025-04-07T03:20:42.463ZS-EPMC101014543705330210.1371/journal.pone.0282732