biostudies-literatureLee MSMinistry of Science, ICT and Future PlanningNational Research Foundation of Korea452-467https://www.ebi.ac.uk/biostudies/studies/S-EPMC10101799biostudies-literatureUnknown55(2)<h4>Purpose</h4>NUT carcinoma (NC) is a solid tumor caused by the rearrangement of NUTM1 that usually develops in midline structures, such as the thorax. No standard treatment has been established despite high lethality. Thus, we investigated whether targeting the junction region of NUTM1 fusion breakpoints could serve as a potential treatment option for NC.<h4>Materials and methods</h4>We designed and evaluated a series of small interfering RNAs (siRNAs) targeting the junction region of BRD4-NUTM1 fusion (B4N), the most common form of NUTM1 fusion. Droplet digital polymerase chain reaction using the blood of patients was also tested to evaluate the treatment responses by the junction sequence of the B4N fusion transcripts.<h4>Results</h4>As expected, the majority of NC fusion types were BCancer research and treatmentCancer-Specific Sequences in the Diagnosis and Treatment of NUT Carcinoma.PMC101017992022R1A2C20063222021R1A2C4002158Yang HChang ESJung KLee SHChoi JJeon YKLakshmi CPark SSung MChoi YLLee MSOh DYSong JYHan JAn SfalseCancer-Specific Sequences in the Diagnosis and Treatment of NUT Carcinoma.<h4>Purpose</h4>NUT carcinoma (NC) is a solid tumor caused by the rearrangement of NUTM1 that usually develops in midline structures, such as the thorax. No standard treatment has been established despite high lethality. Thus, we investigated whether targeting the junction region of NUTM1 fusion breakpoints could serve as a potential treatment option for NC.<h4>Materials and methods</h4>We designed and evaluated a series of small interfering RNAs (siRNAs) targeting the junction region of BRD4-NUTM1 fusion (B4N), the most common form of NUTM1 fusion. Droplet digital polymerase chain reaction using the blood of patients was also tested to evaluate the treatment responses by the junction sequence of the B4N fusion transcripts.<h4>Results</h4>As expected, the majority of NC fusion types were B2023-01-01T00:00:00Z2023 Apr2026-07-14T22:25:27.962Z2025-02-19T04:16:30.994ZS-EPMC101017993626550910.4143/crt.2022.910