biostudies-literatureZhang LBeijing Municipal Natural Science FoundationChinese Academy of Medical Sciences (CAMS) Innovation Fund for Medical Sciencesthe Guangdong Scientific and Technological Foundationthe Nonprofit Central Research Institute Fund of Chinese Academy of Medical SciencesBeijing Municipal Science and Technology Commission ProgramChinese Academy of Medical Sciences (CAMS) Innovation Fund for Medical Sciences (CIFMS)the National Major Science & Technology Project for Control and Prevention of Major Infectious Diseases in Chinathe National Major Science &amp; Technology Project for Control and Prevention of Major Infectious Diseases in ChinaFondation Mérieux231https://www.ebi.ac.uk/biostudies/studies/S-EPMC10103676biostudies-literatureUnknown23(1)<h4>Background</h4>Community-acquired pneumonia (CAP) is a major public health challenge worldwide. However, the aetiological and disease severity-related pathogens associated with CAP in adults in China are not well established based on the detection of both viral and bacterial agents.<h4>Methods</h4>A multicentre, prospective study was conducted involving 10 hospitals located in nine geographical regions in China from 2014 to 2019. Sputum or bronchoalveolar lavage fluid (BALF) samples were collected from each recruited CAP patient. Multiplex real-time PCR and bacteria culture methods were used to detect respiratory pathogens. The association between detected pathogens and CAP severity was evaluated.<h4>Results</h4>Among the 3,403 recruited eligible patients, 462 (13.58%) had severe CAP, BMC infectious diseasesIdentification of priority pathogens for aetiological diagnosis in adults with community-acquired pneumonia in China: a multicentre prospective study.PMC10103676Z1900172019PT310022021-I2M-1-0382020B1111170014Z1911000066191022017ZX10103004Zhang GWen ZWu LQiu HLiu LZhang LWang JWang LRen LWu SZhao FChen YWang XWang YZhao JLi HChen MXiao YChen LChen FYang DSun YLi WLi YGao ZPeng LfalseIdentification of priority pathogens for aetiological diagnosis in adults with community-acquired pneumonia in China: a multicentre prospective study.<h4>Background</h4>Community-acquired pneumonia (CAP) is a major public health challenge worldwide. However, the aetiological and disease severity-related pathogens associated with CAP in adults in China are not well established based on the detection of both viral and bacterial agents.<h4>Methods</h4>A multicentre, prospective study was conducted involving 10 hospitals located in nine geographical regions in China from 2014 to 2019. Sputum or bronchoalveolar lavage fluid (BALF) samples were collected from each recruited CAP patient. Multiplex real-time PCR and bacteria culture methods were used to detect respiratory pathogens. The association between detected pathogens and CAP severity was evaluated.<h4>Results</h4>Among the 3,403 recruited eligible patients, 462 (13.58%) had severe CAP, 2023-01-01T00:00:00Z2023 Apr2025-04-04T23:00:21.84Z2025-04-04T23:00:21.84ZS-EPMC101036763705998710.1186/s12879-023-08166-3