biostudies-literatureUnknownAdelus MLNHLBI NIH HHSNIGMS NIH HHS<h4>Objective</h4>Endothelial cells (ECs), macrophages, and vascular smooth muscle cells (VSMCs) are major cell types in atherosclerosis progression, and heterogeneity in EC sub-phenotypes are becoming increasingly appreciated. Still, studies quantifying EC heterogeneity across whole transcriptomes and epigenomes in both <i>in vitro</i> and <i>in vivo</i> models are lacking.<h4>Approach and results</h4>To create an <i>in vitro</i> dataset to study human EC heterogeneity, multiomic profiling concurrently measuring transcriptomes and accessible chromatin in the same single cells was performed on six distinct primary cultures of human aortic ECs (HAECs). To model pro-inflammatory and activating environments characteristic of the atherosclerotic microenvironment <i>in vitro</i>, HAECs from at bioRxiv : the preprint server for biology2023.04.03.535495https://www.ebi.ac.uk/biostudies/studies/S-EPMC10104082biostudies-literatureSingle cell 'omic profiles of human aortic endothelial cells <i>in vitro</i> and human atherosclerotic lesions <i>ex vivo</i> reveals heterogeneity of endothelial subtype and response to activating perturbations.PMC10104082R35 GM137896T32 HL007249F30 HL162469R01 HL147187Cusanovich DAWhalen MBGolebiewski AKRomanoski CETran BTAdelus MLStolze LKDing JConklin ACfalseSingle cell 'omic profiles of human aortic endothelial cells <i>in vitro</i> and human atherosclerotic lesions <i>ex vivo</i> reveals heterogeneity of endothelial subtype and response to activating perturbations.<h4>Objective</h4>Endothelial cells (ECs), macrophages, and vascular smooth muscle cells (VSMCs) are major cell types in atherosclerosis progression, and heterogeneity in EC sub-phenotypes are becoming increasingly appreciated. Still, studies quantifying EC heterogeneity across whole transcriptomes and epigenomes in both <i>in vitro</i> and <i>in vivo</i> models are lacking.<h4>Approach and results</h4>To create an <i>in vitro</i> dataset to study human EC heterogeneity, multiomic profiling concurrently measuring transcriptomes and accessible chromatin in the same single cells was performed on six distinct primary cultures of human aortic ECs (HAECs). To model pro-inflammatory and activating environments characteristic of the atherosclerotic microenvironment <i>in vitro</i>, HAECs from at 2024-01-01T00:00:00Z2024 Jan2026-05-17T03:13:40.596Z2025-02-19T02:39:14.566ZS-EPMC101040823706641610.1101/2023.04.03.535495