{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["El-Sayed SAE"],"funding":["Ministry of Higher Education, culture mission affairs, Egypt","Ministry of Education, Culture, Sports, Science, and Technology of Japan","Japan Society for the Promotion of Science"],"pagination":["e0284535"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10104287"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(4)"],"pubmed_abstract":["In this study, we designed novel truncated Babesia caballi (B. caballi) recombinant proteins from the previously used B. caballi proteins; 134-Kilodalton Protein (rBC134) and Merozoite Rhoptry 48 Protein (rBC48). Then, we evaluated the diagnostic performance of the newly designed proteins when used as a single antigen or when used as cocktail antigen consists of rBC134 full length (rBC134f) + newly designed rBC48 (rBC48t) or newly designed rBC134 (rBC134t) + rBC48t for the detection of B. caballi infection in horse using indirect enzyme-linked immunosorbent assay (iELISA). We used one dose and a half of each antigen in the cocktail formulas. The serum samples were collected from different endemic areas in addition to the sera collected from horses experimentally infected with B. caballi we"],"journal":["PloS one"],"pubmed_title":["Development of a promising antigenic cocktail for the global detection of Babesia caballi in horse by ELISA."],"pmcid":["PMC10104287"],"funding_grant_id":["P18091"],"pubmed_authors":["Sambuu G","Igarashi I","Ringo AE","Rizk MA","Baghdadi HB","Nugraha AB","El-Sayed SAE"],"additional_accession":[]},"is_claimable":false,"name":"Development of a promising antigenic cocktail for the global detection of Babesia caballi in horse by ELISA.","description":"In this study, we designed novel truncated Babesia caballi (B. caballi) recombinant proteins from the previously used B. caballi proteins; 134-Kilodalton Protein (rBC134) and Merozoite Rhoptry 48 Protein (rBC48). Then, we evaluated the diagnostic performance of the newly designed proteins when used as a single antigen or when used as cocktail antigen consists of rBC134 full length (rBC134f) + newly designed rBC48 (rBC48t) or newly designed rBC134 (rBC134t) + rBC48t for the detection of B. caballi infection in horse using indirect enzyme-linked immunosorbent assay (iELISA). We used one dose and a half of each antigen in the cocktail formulas. The serum samples were collected from different endemic areas in addition to the sera collected from horses experimentally infected with B. caballi we","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2025-04-05T13:15:04.244Z","creation":"2025-04-05T13:15:04.244Z"},"accession":"S-EPMC10104287","cross_references":{"pubmed":["37058508"],"doi":["10.1371/journal.pone.0284535"]}}