{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Matsuda Y"],"funding":["MEXT | Japan Society for the Promotion of Science","Ministry of Health, Labour and Welfare","U.S. Department of Energy"],"pagination":["e2216550120"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10104525"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["120(15)"],"pubmed_abstract":["Spectra and frequencies of spontaneous and X-ray-induced somatic mutations were revealed with mouse long-term hematopoietic stem cells (LT-HSCs) by whole-genome sequencing of clonal cell populations propagated in vitro from single isolated LT-HSCs. SNVs and small indels were the most common types of somatic mutations, and increased up to twofold to threefold by whole-body X-irradiation. Base substitution patterns in the SNVs suggested a role of reactive oxygen species in radiation mutagenesis, and signature analysis of single base substitutions (SBS) revealed a dose-dependent increase of SBS40. Most of spontaneous small deletions were shrinkage of tandem repeats, and X-irradiation specifically induced small deletions out of tandem repeats (non-repeat deletions). Presence of microhomology s"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Spectra and characteristics of somatic mutations induced by ionizing radiation in hematopoietic stem cells."],"pmcid":["PMC10104525"],"funding_grant_id":["22K12388","Not applicable","19K12338"],"pubmed_authors":["Toshishige M","Kajimura J","Kato N","Satoh Y","Hayashi T","Noda A","Tanabe O","Hamasaki K","Yoshida K","Matsuda Y","Uchimura A"],"additional_accession":[]},"is_claimable":false,"name":"Spectra and characteristics of somatic mutations induced by ionizing radiation in hematopoietic stem cells.","description":"Spectra and frequencies of spontaneous and X-ray-induced somatic mutations were revealed with mouse long-term hematopoietic stem cells (LT-HSCs) by whole-genome sequencing of clonal cell populations propagated in vitro from single isolated LT-HSCs. SNVs and small indels were the most common types of somatic mutations, and increased up to twofold to threefold by whole-body X-irradiation. Base substitution patterns in the SNVs suggested a role of reactive oxygen species in radiation mutagenesis, and signature analysis of single base substitutions (SBS) revealed a dose-dependent increase of SBS40. Most of spontaneous small deletions were shrinkage of tandem repeats, and X-irradiation specifically induced small deletions out of tandem repeats (non-repeat deletions). Presence of microhomology s","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2025-04-04T12:44:41.87Z","creation":"2025-04-04T12:44:41.87Z"},"accession":"S-EPMC10104525","cross_references":{"pubmed":["37018193"],"doi":["10.1073/pnas.2216550120"]}}