{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zappia MP"],"funding":["HHS | NIH | National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["e2220770120"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10104548"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["120(15)"],"pubmed_abstract":["The canonical role of the transcription factor E2F is to control the expression of cell cycle genes by binding to the E2F sites in their promoters. However, the list of putative E2F target genes is extensive and includes many metabolic genes, yet the significance of E2F in controlling the expression of these genes remains largely unknown. Here, we used the CRISPR/Cas9 technology to introduce point mutations in the E2F sites upstream of five endogenous metabolic genes in <i>Drosophila melanogaster</i>. We found that the impact of these mutations on both the recruitment of E2F and the expression of the target genes varied, with the glycolytic gene, <i>Phosphoglycerate kinase</i> (<i>Pgk)</i>, being mostly affected. The loss of E2F regulation on the <i>Pgk</i> gene led to a decrease in glycol"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["E2F regulation of the <i>Phosphoglycerate kinase</i> gene is functionally important in <i>Drosophila</i> development."],"pmcid":["PMC10104548"],"funding_grant_id":["R35GM131707","R35 GM131707"],"pubmed_authors":["Islam ABMMK","Kwon YJ","Lee HM","Frolov MV","Liseth I","Westacott A","Zappia MP","Kim J"],"additional_accession":[]},"is_claimable":false,"name":"E2F regulation of the <i>Phosphoglycerate kinase</i> gene is functionally important in <i>Drosophila</i> development.","description":"The canonical role of the transcription factor E2F is to control the expression of cell cycle genes by binding to the E2F sites in their promoters. However, the list of putative E2F target genes is extensive and includes many metabolic genes, yet the significance of E2F in controlling the expression of these genes remains largely unknown. Here, we used the CRISPR/Cas9 technology to introduce point mutations in the E2F sites upstream of five endogenous metabolic genes in <i>Drosophila melanogaster</i>. We found that the impact of these mutations on both the recruitment of E2F and the expression of the target genes varied, with the glycolytic gene, <i>Phosphoglycerate kinase</i> (<i>Pgk)</i>, being mostly affected. The loss of E2F regulation on the <i>Pgk</i> gene led to a decrease in glycol","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2025-04-22T15:55:16.09Z","creation":"2025-04-06T01:35:10.093Z"},"accession":"S-EPMC10104548","cross_references":{"pubmed":["37011211"],"doi":["10.1073/pnas.2220770120"]}}