{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["120(15)"],"submitter":["Izadi A"],"pubmed_abstract":["Antibodies play a central role in the immune defense against SARS-CoV-2. Emerging evidence has shown that nonneutralizing antibodies are important for immune defense through Fc-mediated effector functions. Antibody subclass is known to affect downstream Fc function. However, whether the antibody subclass plays a role in anti-SARS-CoV-2 immunity remains unclear. Here, we subclass-switched eight human IgG1 anti-spike monoclonal antibodies (mAbs) to the IgG3 subclass by exchanging their constant domains. The IgG3 mAbs exhibited altered avidities to the spike protein and more potent Fc-mediated phagocytosis and complement activation than their IgG1 counterparts. Moreover, combining mAbs into oligoclonal cocktails led to enhanced Fc- and complement receptor-mediated phagocytosis, superior to ev"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pagination":["e2217590120"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10104557"],"repository":["biostudies-literature"],"pubmed_title":["Subclass-switched anti-spike IgG3 oligoclonal cocktails strongly enhance Fc-mediated opsonization."],"pmcid":["PMC10104557"],"pubmed_authors":["Ohlin M","Larsson O","Elder E","Appelberg S","Izadi A","Wrighton S","Wendel-Hansen V","Bahnan W","Valsjo M","Godzwon M","Olofsson B","Hailu A","Schmidt T","Nordenfelt P","Soderlund-Strand A"],"additional_accession":[]},"is_claimable":false,"name":"Subclass-switched anti-spike IgG3 oligoclonal cocktails strongly enhance Fc-mediated opsonization.","description":"Antibodies play a central role in the immune defense against SARS-CoV-2. Emerging evidence has shown that nonneutralizing antibodies are important for immune defense through Fc-mediated effector functions. Antibody subclass is known to affect downstream Fc function. However, whether the antibody subclass plays a role in anti-SARS-CoV-2 immunity remains unclear. Here, we subclass-switched eight human IgG1 anti-spike monoclonal antibodies (mAbs) to the IgG3 subclass by exchanging their constant domains. The IgG3 mAbs exhibited altered avidities to the spike protein and more potent Fc-mediated phagocytosis and complement activation than their IgG1 counterparts. Moreover, combining mAbs into oligoclonal cocktails led to enhanced Fc- and complement receptor-mediated phagocytosis, superior to ev","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2025-04-21T21:57:18.56Z","creation":"2025-04-05T18:29:01.506Z"},"accession":"S-EPMC10104557","cross_references":{"pubmed":["37011197"],"doi":["10.1073/pnas.2217590120"]}}