biostudies-literatureAsano-Inami EMEXT | Japan Society for the Promotion of Science415https://www.ebi.ac.uk/biostudies/studies/S-EPMC10104854biostudies-literatureUnknown6(1)Stress granules (SGs) are dynamic, non-membranous structures composed of non-translating mRNAs and various proteins and play critical roles in cell survival under stressed conditions. Extensive proteomics analyses have been performed to identify proteins in SGs; however, the molecular functions of these components in SG formation remain unclear. In this report, we show that ubiquitin-associated protein 2-like (UBAP2L) is a crucial component of SGs. UBAP2L localized to SGs in response to various stresses, and its depletion significantly suppressed SG organization. Proteomics and RNA sequencing analyses found that UBAP2L formed a protein-RNA complex with Ras-GTP-activating protein SH3 domain binding protein 1 (G3BP1) and small nucleolar RNAs (snoRNAs). In vitro binding analysis demonstrated Communications biologyThe association of UBAP2L and G3BP1 mediated by small nucleolar RNA is essential for stress granule formation.PMC1010485421H03075Hamaguchi TKajiyama HHyodo TYokoi AAsano-Inami ESugiyama MfalseThe association of UBAP2L and G3BP1 mediated by small nucleolar RNA is essential for stress granule formation.Stress granules (SGs) are dynamic, non-membranous structures composed of non-translating mRNAs and various proteins and play critical roles in cell survival under stressed conditions. Extensive proteomics analyses have been performed to identify proteins in SGs; however, the molecular functions of these components in SG formation remain unclear. In this report, we show that ubiquitin-associated protein 2-like (UBAP2L) is a crucial component of SGs. UBAP2L localized to SGs in response to various stresses, and its depletion significantly suppressed SG organization. Proteomics and RNA sequencing analyses found that UBAP2L formed a protein-RNA complex with Ras-GTP-activating protein SH3 domain binding protein 1 (G3BP1) and small nucleolar RNAs (snoRNAs). In vitro binding analysis demonstrated 2023-01-01T00:00:00Z2023 Apr2025-04-04T23:00:27.218Z2025-04-04T23:00:27.218ZS-EPMC101048543705980310.1038/s42003-023-04754-w