biostudies-literatureBignon YSwiss National Science Foundatione167133https://www.ebi.ac.uk/biostudies/studies/S-EPMC10104904biostudies-literatureUnknown133(8)Circadian rhythmicity in renal function suggests rhythmic adaptations in renal metabolism. To decipher the role of the circadian clock in renal metabolism, we studied diurnal changes in renal metabolic pathways using integrated transcriptomic, proteomic, and metabolomic analysis performed on control mice and mice with an inducible deletion of the circadian clock regulator Bmal1 in the renal tubule (cKOt). With this unique resource, we demonstrated that approximately 30% of RNAs, approximately 20% of proteins, and approximately 20% of metabolites are rhythmic in the kidneys of control mice. Several key metabolic pathways, including NAD+ biosynthesis, fatty acid transport, carnitine shuttle, and β-oxidation, displayed impairments in kidneys of cKOt mice, resulting in perturbed mitochondrial The Journal of clinical investigationMultiomics reveals multilevel control of renal and systemic metabolism by the renal tubular circadian clock.PMC10104904310030-188499Gotz LWeger MBignon YCenteno GDurussel FGachon FLagarrigue SPradervand SAnsermet CQuadroni MAmati FIbberson MWigger LWeger BDFirsov DfalseMultiomics reveals multilevel control of renal and systemic metabolism by the renal tubular circadian clock.Circadian rhythmicity in renal function suggests rhythmic adaptations in renal metabolism. To decipher the role of the circadian clock in renal metabolism, we studied diurnal changes in renal metabolic pathways using integrated transcriptomic, proteomic, and metabolomic analysis performed on control mice and mice with an inducible deletion of the circadian clock regulator Bmal1 in the renal tubule (cKOt). With this unique resource, we demonstrated that approximately 30% of RNAs, approximately 20% of proteins, and approximately 20% of metabolites are rhythmic in the kidneys of control mice. Several key metabolic pathways, including NAD+ biosynthesis, fatty acid transport, carnitine shuttle, and β-oxidation, displayed impairments in kidneys of cKOt mice, resulting in perturbed mitochondrial 2023-01-01T00:00:00Z2023 Apr2026-05-28T08:06:13.267Z2025-04-25T17:11:01.967ZS-EPMC101049043686251110.1172/JCI167133