{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14(1)"],"submitter":["Sahoo A"],"pubmed_abstract":["<h4>Background</h4>Mesenchymal stem cells (MSCs) have been proven to prevent and clear corneal scarring and limbal stem cell deficiency. However, using animal-derived serum in a culture medium raises the ethical and regulatory bar. This study aims to expand and characterize human limbus-derived stromal/mesenchymal stem cells (hLMSCs) for the first time in vitro in the xeno-free medium.<h4>Methods</h4>Limbal tissue was obtained from therapeutic grade corneoscleral rims and subjected to explant culture till tertiary passage in media with and without serum (STEM MACS XF; SM), to obtain pure hLMSCs. Population doubling time, cell proliferation, expression of phenotypic markers, tri-lineage differentiation, colony-forming potential and gene expression analysis were carried out to assess the ret"],"journal":["Stem cell research & therapy"],"pagination":["89"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10105964"],"repository":["biostudies-literature"],"pubmed_title":["Expansion and characterization of human limbus-derived stromal/mesenchymal stem cells in xeno-free medium for therapeutic applications."],"pmcid":["PMC10105964"],"pubmed_authors":["Sahoo A","Damala M","Prasad D","Basu S","Jaffet J","Singh V"],"additional_accession":[]},"is_claimable":false,"name":"Expansion and characterization of human limbus-derived stromal/mesenchymal stem cells in xeno-free medium for therapeutic applications.","description":"<h4>Background</h4>Mesenchymal stem cells (MSCs) have been proven to prevent and clear corneal scarring and limbal stem cell deficiency. However, using animal-derived serum in a culture medium raises the ethical and regulatory bar. This study aims to expand and characterize human limbus-derived stromal/mesenchymal stem cells (hLMSCs) for the first time in vitro in the xeno-free medium.<h4>Methods</h4>Limbal tissue was obtained from therapeutic grade corneoscleral rims and subjected to explant culture till tertiary passage in media with and without serum (STEM MACS XF; SM), to obtain pure hLMSCs. Population doubling time, cell proliferation, expression of phenotypic markers, tri-lineage differentiation, colony-forming potential and gene expression analysis were carried out to assess the ret","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2026-07-14T20:14:06.522Z","creation":"2025-04-04T20:46:12.853Z"},"accession":"S-EPMC10105964","cross_references":{"pubmed":["37061739"],"doi":["10.1186/s13287-023-03299-3"]}}