biostudies-literatureUnknown11(1)Macdonald S<h4>Purpose</h4>To investigate the effect of IV fluid resuscitation on endothelial glycocalyx (EG) shedding and activation of the vascular endothelium and inflammation.<h4>Materials and methods</h4>A planned biomarker sub-study of the REFRESH trial in which emergency department (ED) patients) with suspected sepsis and hypotension were randomised to a restricted fluid/early vasopressor regimen or IV fluid resuscitation with later vasopressors if required (usual care). Blood samples were collected at randomisation (T0) and at 3 h (T3), 6 h (T6)- and 24 h (T24) for measurement of a range of biomarkers if EG shedding, endothelial cell activation and inflammation.<h4>Results</h4>Blood samples were obtained in 95 of 99 enrolled patients (46 usual care, 49 restricted fluid). Differences in the chIntensive care medicine experimental21https://www.ebi.ac.uk/biostudies/studies/S-EPMC10106534biostudies-literatureEffect of intravenous fluid volume on biomarkers of endothelial glycocalyx shedding and inflammation during initial resuscitation of sepsis.PMC10106534Leonard AVlad IMacdonald SKeijzers GMukherjee AHarley AHazelwood SREFRESH Trial InvestigatorsFraser JShapiro NIWinearls JTaylor DBurrows SAscencio-Lane JCMcCutcheon DHibbs MO'Donoghue HFatovich DSmart LWibrow BAnstey MLitton EBurcham JBellomo RArendts GCooper DKinnear FBosio EBrown SfalseEffect of intravenous fluid volume on biomarkers of endothelial glycocalyx shedding and inflammation during initial resuscitation of sepsis.<h4>Purpose</h4>To investigate the effect of IV fluid resuscitation on endothelial glycocalyx (EG) shedding and activation of the vascular endothelium and inflammation.<h4>Materials and methods</h4>A planned biomarker sub-study of the REFRESH trial in which emergency department (ED) patients) with suspected sepsis and hypotension were randomised to a restricted fluid/early vasopressor regimen or IV fluid resuscitation with later vasopressors if required (usual care). Blood samples were collected at randomisation (T0) and at 3 h (T3), 6 h (T6)- and 24 h (T24) for measurement of a range of biomarkers if EG shedding, endothelial cell activation and inflammation.<h4>Results</h4>Blood samples were obtained in 95 of 99 enrolled patients (46 usual care, 49 restricted fluid). Differences in the ch2023-01-01T00:00:00Z2023 Apr2025-04-22T10:03:28.996Z2025-04-05T23:22:46.319ZS-EPMC101065343706276910.1186/s40635-023-00508-4