{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang C"],"funding":["National Natural Science Foundation of China"],"pagination":["e111472"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10106985"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["42(8)"],"pubmed_abstract":["For shade-intolerant plants, changes in light quality through competition from neighbors trigger shade avoidance syndrome (SAS): a series of morphological and physiological adaptations that are ultimately detrimental to plant health and crop yield. Phytochrome-interacting factor 7 (PIF7) is a major transcriptional regulator of SAS in Arabidopsis; however, how it regulates gene expression is not fully understood. Here, we show that PIF7 directly interacts with the histone chaperone anti-silencing factor 1 (ASF1). The ASF1-deprived asf1ab mutant showed defective shade-induced hypocotyl elongation. Histone regulator homolog A (HIRA), which mediates deposition of the H3.3 variant into chromatin, is also involved in SAS. RNA/ChIP-sequencing analyses identified the role of ASF1 in the direct regulation of a subset of PIF7 target genes. Furthermore, shade-elicited gene activation is accompanied by H3.3 enrichment, which is mediated by the PIF7-ASF1-HIRA regulatory module. Collectively, our data reveal that PIF7 recruits ASF1-HIRA to increase H3.3 incorporation into chromatin to promote gene transcription, thus enabling plants to effectively respond to environmental shade."],"journal":["The EMBO journal"],"pubmed_title":["PIF7-mediated epigenetic reprogramming promotes the transcriptional response to shade in Arabidopsis."],"pmcid":["PMC10106985"],"funding_grant_id":["2017YFA0503800"],"pubmed_authors":["Huang S","Zhou N","Xie Y","Jiang W","Zeng Y","Shen WH","Yang C","Li L","Zhu T"],"additional_accession":[]},"is_claimable":false,"name":"PIF7-mediated epigenetic reprogramming promotes the transcriptional response to shade in Arabidopsis.","description":"For shade-intolerant plants, changes in light quality through competition from neighbors trigger shade avoidance syndrome (SAS): a series of morphological and physiological adaptations that are ultimately detrimental to plant health and crop yield. Phytochrome-interacting factor 7 (PIF7) is a major transcriptional regulator of SAS in Arabidopsis; however, how it regulates gene expression is not fully understood. Here, we show that PIF7 directly interacts with the histone chaperone anti-silencing factor 1 (ASF1). The ASF1-deprived asf1ab mutant showed defective shade-induced hypocotyl elongation. Histone regulator homolog A (HIRA), which mediates deposition of the H3.3 variant into chromatin, is also involved in SAS. RNA/ChIP-sequencing analyses identified the role of ASF1 in the direct regulation of a subset of PIF7 target genes. Furthermore, shade-elicited gene activation is accompanied by H3.3 enrichment, which is mediated by the PIF7-ASF1-HIRA regulatory module. Collectively, our data reveal that PIF7 recruits ASF1-HIRA to increase H3.3 incorporation into chromatin to promote gene transcription, thus enabling plants to effectively respond to environmental shade.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2026-06-26T03:24:25.382Z","creation":"2025-04-06T14:33:20.259Z"},"accession":"S-EPMC10106985","cross_references":{"pubmed":["36912149"],"doi":["10.15252/embj.2022111472"]}}