{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Haskett TL"],"funding":["Royal Commission for the Exhibition of 1851","Biotechnology and Biological Sciences Research Council","National Science Foundation"],"pagination":["383-396"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10107442"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(2)"],"pubmed_abstract":["Engineering signalling between plants and microbes could be exploited to establish host-specificity between plant-growth-promoting bacteria and target crops in the environment. We previously engineered rhizopine-signalling circuitry facilitating exclusive signalling between rhizopine-producing (RhiP) plants and model bacterial strains. Here, we conduct an in-depth analysis of rhizopine-inducible expression in bacteria. We characterize two rhizopine-inducible promoters and explore the bacterial host-range of rhizopine biosensor plasmids. By tuning the expression of rhizopine uptake genes, we also construct a new biosensor plasmid pSIR05 that has minimal impact on host cell growth in vitro and exhibits markedly improved stability of expression in situ on RhiP barley roots compared to the pre"],"journal":["Environmental microbiology"],"pubmed_title":["Rhizopine biosensors for plant-dependent control of bacterial gene expression."],"pmcid":["PMC10107442"],"funding_grant_id":["RF‐2019‐100238","NSF‐1331098","BB/T006722/1","2107666","BB/M011224/1"],"pubmed_authors":["Bastholm TR","Paramasivan P","Poole PS","Green P","Jorrin B","Mendes MD","Ramsay JP","Geddes BA","Oldroyd GED","Haskett TL","Chitnavis S","Knights HE"],"additional_accession":[]},"is_claimable":false,"name":"Rhizopine biosensors for plant-dependent control of bacterial gene expression.","description":"Engineering signalling between plants and microbes could be exploited to establish host-specificity between plant-growth-promoting bacteria and target crops in the environment. We previously engineered rhizopine-signalling circuitry facilitating exclusive signalling between rhizopine-producing (RhiP) plants and model bacterial strains. Here, we conduct an in-depth analysis of rhizopine-inducible expression in bacteria. We characterize two rhizopine-inducible promoters and explore the bacterial host-range of rhizopine biosensor plasmids. By tuning the expression of rhizopine uptake genes, we also construct a new biosensor plasmid pSIR05 that has minimal impact on host cell growth in vitro and exhibits markedly improved stability of expression in situ on RhiP barley roots compared to the pre","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-26T05:01:59.176Z","creation":"2025-04-06T11:20:40.214Z"},"accession":"S-EPMC10107442","cross_references":{"pubmed":["36428208"],"doi":["10.1111/1462-2920.16288"]}}