biostudies-literatureMorgan JResearch FoundationMCIN/AEI/10.13039/501100011033European UnionHorizon 2020 Framework ProgrammeScience Foundation IrelandIrish Research Council for Science, Engineering and TechnologyASCRS Research FoundationHorizon 2020European CommissionH2020 Marie Skłodowska-Curie ActionsEuropean Regional Development Fund511-523https://www.ebi.ac.uk/biostudies/studies/S-EPMC10108015biostudies-literatureUnknown64(2)<h4>Objective</h4>The P2X7 receptor (P2X7R) is an important contributor to neuroinflammation, responding to extracellularly released adenosine triphosphate. Expression of the P2X7R is increased in the brain in experimental and human epilepsy, and genetic or pharmacologic targeting of the receptor can reduce seizure frequency and severity in preclinical models. Experimentally induced seizures also increase levels of the P2X7R in blood. Here, we tested ¹⁸ F-JNJ-64413739, a positron emission tomography (PET) P2X7R antagonist, as a potential noninvasive biomarker of seizure-damage and epileptogenesis.<h4>Methods</h4>Status epilepticus was induced via an intra-amygdala microinjection of kainic acid. Static PET studies (30 min duration, initiated 30 min after tracer administration) weEpilepsiaIncreased uptake of the P2X7 receptor radiotracer ¹⁸ F-JNJ-64413739 in the brain and peripheral organs according to the severity of status epilepticus in male mice.PMC10108015766124MDM-2017-0720GOIPD/2020/86516/RC/3948884956PID2020-117656RB-I0017/CDA/4708Morgan JLeister HGomez-Vallejo VEngel TAlves MHenshall DCSmith JVisekruna AMelia CBhattacharya AMenendez Mendez ABaz ZCeusters MNicke AMoreno OLlop JfalseIncreased uptake of the P2X7 receptor radiotracer ¹⁸ F-JNJ-64413739 in the brain and peripheral organs according to the severity of status epilepticus in male mice.<h4>Objective</h4>The P2X7 receptor (P2X7R) is an important contributor to neuroinflammation, responding to extracellularly released adenosine triphosphate. Expression of the P2X7R is increased in the brain in experimental and human epilepsy, and genetic or pharmacologic targeting of the receptor can reduce seizure frequency and severity in preclinical models. Experimentally induced seizures also increase levels of the P2X7R in blood. Here, we tested ¹⁸ F-JNJ-64413739, a positron emission tomography (PET) P2X7R antagonist, as a potential noninvasive biomarker of seizure-damage and epileptogenesis.<h4>Methods</h4>Status epilepticus was induced via an intra-amygdala microinjection of kainic acid. Static PET studies (30 min duration, initiated 30 min after tracer administration) we2023-01-01T00:00:00Z2023 Feb2026-05-02T11:19:11.188Z2024-11-06T21:28:13.4ZS-EPMC101080153650770810.1111/epi.17484