{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Stout AJ"],"funding":["NIBIB NIH HHS","U.S. Department of Agriculture","New Harvest","National Institutes of Health","Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology"],"pagination":["122092"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10111969"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["296"],"pubmed_abstract":["The development of cost-effective serum-free media is essential for the economic viability of cultured meat. A key challenge facing this goal is the high-cost of recombinant albumin which is necessary in many serum-free media formulations, including a recently developed serum-free medium for bovine satellite cell (BSC) culture termed Beefy-9. Here we alter Beefy-9 by replacing recombinant albumin with rapeseed protein isolate (RPI), a bulk-protein solution obtained from agricultural waste through alkali extraction (pH 12.5), isoelectric protein precipitation (pH 4.5), dissolution of physiologically soluble proteins (pH 7.2), and concentration of proteins through 3 kDa ultrafiltration. This new medium, termed Beefy-R, was then used to culture BSCs over four passages, during which cells grew"],"journal":["Biomaterials"],"pubmed_title":["A Beefy-R culture medium: Replacing albumin with rapeseed protein isolates."],"pmcid":["PMC10111969"],"funding_grant_id":["P41 EB027062","P41 EB002520","P41EB002520","2021-69012-35978"],"pubmed_authors":["Kaplan DL","Rittenberg ML","Stout AJ","Mirliani AB","Shub M","Saad MK","Dolgin J"],"additional_accession":[]},"is_claimable":false,"name":"A Beefy-R culture medium: Replacing albumin with rapeseed protein isolates.","description":"The development of cost-effective serum-free media is essential for the economic viability of cultured meat. A key challenge facing this goal is the high-cost of recombinant albumin which is necessary in many serum-free media formulations, including a recently developed serum-free medium for bovine satellite cell (BSC) culture termed Beefy-9. Here we alter Beefy-9 by replacing recombinant albumin with rapeseed protein isolate (RPI), a bulk-protein solution obtained from agricultural waste through alkali extraction (pH 12.5), isoelectric protein precipitation (pH 4.5), dissolution of physiologically soluble proteins (pH 7.2), and concentration of proteins through 3 kDa ultrafiltration. This new medium, termed Beefy-R, was then used to culture BSCs over four passages, during which cells grew","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2026-06-02T22:47:32.855Z","creation":"2026-04-21T03:13:36.319Z"},"accession":"S-EPMC10111969","cross_references":{"pubmed":["36965281"],"doi":["10.1016/j.biomaterials.2023.122092"]}}