biostudies-literatureStout AJNIBIB NIH HHSU.S. Department of AgricultureNew HarvestNational Institutes of HealthKoch Institute for Integrative Cancer Research, Massachusetts Institute of Technology122092https://www.ebi.ac.uk/biostudies/studies/S-EPMC10111969biostudies-literatureUnknown296The development of cost-effective serum-free media is essential for the economic viability of cultured meat. A key challenge facing this goal is the high-cost of recombinant albumin which is necessary in many serum-free media formulations, including a recently developed serum-free medium for bovine satellite cell (BSC) culture termed Beefy-9. Here we alter Beefy-9 by replacing recombinant albumin with rapeseed protein isolate (RPI), a bulk-protein solution obtained from agricultural waste through alkali extraction (pH 12.5), isoelectric protein precipitation (pH 4.5), dissolution of physiologically soluble proteins (pH 7.2), and concentration of proteins through 3 kDa ultrafiltration. This new medium, termed Beefy-R, was then used to culture BSCs over four passages, during which cells grewBiomaterialsA Beefy-R culture medium: Replacing albumin with rapeseed protein isolates.PMC10111969P41 EB027062P41 EB002520P41EB0025202021-69012-35978Kaplan DLRittenberg MLStout AJMirliani ABShub MSaad MKDolgin JfalseA Beefy-R culture medium: Replacing albumin with rapeseed protein isolates.The development of cost-effective serum-free media is essential for the economic viability of cultured meat. A key challenge facing this goal is the high-cost of recombinant albumin which is necessary in many serum-free media formulations, including a recently developed serum-free medium for bovine satellite cell (BSC) culture termed Beefy-9. Here we alter Beefy-9 by replacing recombinant albumin with rapeseed protein isolate (RPI), a bulk-protein solution obtained from agricultural waste through alkali extraction (pH 12.5), isoelectric protein precipitation (pH 4.5), dissolution of physiologically soluble proteins (pH 7.2), and concentration of proteins through 3 kDa ultrafiltration. This new medium, termed Beefy-R, was then used to culture BSCs over four passages, during which cells grew2023-01-01T00:00:00Z2023 May2026-06-02T22:47:32.855Z2026-04-21T03:13:36.319ZS-EPMC101119693696528110.1016/j.biomaterials.2023.122092