{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Darshi M"],"funding":["National Center for Advancing Translational Sciences","Juvenile Diabetes Research Foundation United States of America","NCATS NIH HHS","NIDDK NIH HHS","The University of Texas Health Science Center at San Antonio","National Institutes of Health","U.S. Department of Veterans Affairs","NIGMS NIH HHS"],"pagination":["106462"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10119590"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(4)"],"pubmed_abstract":["The Crabtree effect is defined as a rapid glucose-induced repression of mitochondrial oxidative metabolism and has been described in yeasts and tumor cells. Using plate-based respirometry, we identified the Crabtree effect in normal (non-tumor) kidney proximal tubule epithelial cells (PTEC) but not in other kidney cells (podocytes or mesangial cells) or mammalian cells (C2C12 myoblasts). Glucose-induced repression of respiration was prevented by reducing glycolysis at the proximal step with 2-deoxyglucose and partially reversed by pyruvate. The late-stage glycolytic intermediates glyceraldehyde 3-phosphate, 3-phosphoglycerate, and phosphoenolpyruvate, but not the early-stage glycolytic intermediates or lactate, inhibited respiration in permeabilized PTEC and kidney cortex mitochondria, mim"],"journal":["iScience"],"pubmed_title":["Crabtree effect in kidney proximal tubule cells via late-stage glycolytic intermediates."],"pmcid":["PMC10119590"],"funding_grant_id":["P30 DK089503","P30 DK081943","5F30DK121463","T32 GM007863","P30 DK89503","TL1 TR002647","R24DK082841"],"pubmed_authors":["Saliba A","Darshi M","Tumova J","Baek J","Pennathur S","Kim J","Sharma K"],"additional_accession":[]},"is_claimable":false,"name":"Crabtree effect in kidney proximal tubule cells via late-stage glycolytic intermediates.","description":"The Crabtree effect is defined as a rapid glucose-induced repression of mitochondrial oxidative metabolism and has been described in yeasts and tumor cells. Using plate-based respirometry, we identified the Crabtree effect in normal (non-tumor) kidney proximal tubule epithelial cells (PTEC) but not in other kidney cells (podocytes or mesangial cells) or mammalian cells (C2C12 myoblasts). Glucose-induced repression of respiration was prevented by reducing glycolysis at the proximal step with 2-deoxyglucose and partially reversed by pyruvate. The late-stage glycolytic intermediates glyceraldehyde 3-phosphate, 3-phosphoglycerate, and phosphoenolpyruvate, but not the early-stage glycolytic intermediates or lactate, inhibited respiration in permeabilized PTEC and kidney cortex mitochondria, mim","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2026-05-28T16:50:42.923Z","creation":"2025-02-19T04:16:03.698Z"},"accession":"S-EPMC10119590","cross_references":{"pubmed":["37091239"],"doi":["10.1016/j.isci.2023.106462"]}}