{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Cankar K"],"funding":["H2020 LEIT Biotechnology","Funda??o para a Ci?ncia e a Tecnologia","European Regional Development Fund"],"pubmed_abstract":["For several sesquiterpene lactones (STLs) found in Asteraceae plants, very interesting biomedical activities have been demonstrated. Chicory roots accumulate the guaianolide STLs 8-deoxylactucin, lactucin, and lactucopicrin predominantly in oxalated forms in the latex. In this work, a supercritical fluid extract fraction of chicory STLs containing 8-deoxylactucin and 11β,13-dihydro-8-deoxylactucin was shown to have anti-inflammatory activity in an inflamed intestinal mucosa model. To increase the accumulation of these two compounds in chicory taproots, the lactucin synthase that takes 8-deoxylactucin as the substrate for the regiospecific hydroxylation to generate lactucin needs to be inactivated. Three candidate cytochrome P450 enzymes of the CYP71 clan were identified in chicory. Their t"],"journal":["Journal of agricultural and food chemistry"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10119987"],"repository":["biostudies-literature"],"pubmed_title":["Lactucin Synthase Inactivation Boosts the Accumulation of Anti-inflammatory 8-Deoxylactucin and Its Derivatives in Chicory (<i>Cichorium intybus</i> L.)."],"pmcid":["PMC10119987"],"funding_grant_id":["760891","CEECIND/ 04801/2017","LISBOA-01-0145-FEDER-007344","SFRH/BD/145551/2019"],"pubmed_authors":["Sevenier R","Fernandez N","Santos CN","Cankar K","Schipper B","Bosch D","Hakkert JC","Vahabi K","Tissier A","Baixinho JP","Bundock P","Serra AT","Matos MS","Papastolopoulou C"],"additional_accession":[]},"is_claimable":false,"name":"Lactucin Synthase Inactivation Boosts the Accumulation of Anti-inflammatory 8-Deoxylactucin and Its Derivatives in Chicory (<i>Cichorium intybus</i> L.).","description":"For several sesquiterpene lactones (STLs) found in Asteraceae plants, very interesting biomedical activities have been demonstrated. Chicory roots accumulate the guaianolide STLs 8-deoxylactucin, lactucin, and lactucopicrin predominantly in oxalated forms in the latex. In this work, a supercritical fluid extract fraction of chicory STLs containing 8-deoxylactucin and 11β,13-dihydro-8-deoxylactucin was shown to have anti-inflammatory activity in an inflamed intestinal mucosa model. To increase the accumulation of these two compounds in chicory taproots, the lactucin synthase that takes 8-deoxylactucin as the substrate for the regiospecific hydroxylation to generate lactucin needs to be inactivated. Three candidate cytochrome P450 enzymes of the CYP71 clan were identified in chicory. Their t","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2026-05-28T14:00:32.845Z","creation":"2025-02-19T04:16:26.448Z"},"accession":"S-EPMC10119987","cross_references":{"pubmed":["37036799"],"doi":["10.1021/acs.jafc.2c08959"]}}