biostudies-literatureUnknownCankar KH2020 LEIT BiotechnologyFunda??o para a Ci?ncia e a TecnologiaEuropean Regional Development FundFor several sesquiterpene lactones (STLs) found in Asteraceae plants, very interesting biomedical activities have been demonstrated. Chicory roots accumulate the guaianolide STLs 8-deoxylactucin, lactucin, and lactucopicrin predominantly in oxalated forms in the latex. In this work, a supercritical fluid extract fraction of chicory STLs containing 8-deoxylactucin and 11β,13-dihydro-8-deoxylactucin was shown to have anti-inflammatory activity in an inflamed intestinal mucosa model. To increase the accumulation of these two compounds in chicory taproots, the lactucin synthase that takes 8-deoxylactucin as the substrate for the regiospecific hydroxylation to generate lactucin needs to be inactivated. Three candidate cytochrome P450 enzymes of the CYP71 clan were identified in chicory. Their tJournal of agricultural and food chemistryhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC10119987biostudies-literatureLactucin Synthase Inactivation Boosts the Accumulation of Anti-inflammatory 8-Deoxylactucin and Its Derivatives in Chicory (<i>Cichorium intybus</i> L.).PMC10119987760891CEECIND/ 04801/2017LISBOA-01-0145-FEDER-007344SFRH/BD/145551/2019Sevenier RFernandez NSantos CNCankar KSchipper BBosch DHakkert JCVahabi KTissier ABaixinho JPBundock PSerra ATMatos MSPapastolopoulou CfalseLactucin Synthase Inactivation Boosts the Accumulation of Anti-inflammatory 8-Deoxylactucin and Its Derivatives in Chicory (<i>Cichorium intybus</i> L.).For several sesquiterpene lactones (STLs) found in Asteraceae plants, very interesting biomedical activities have been demonstrated. Chicory roots accumulate the guaianolide STLs 8-deoxylactucin, lactucin, and lactucopicrin predominantly in oxalated forms in the latex. In this work, a supercritical fluid extract fraction of chicory STLs containing 8-deoxylactucin and 11β,13-dihydro-8-deoxylactucin was shown to have anti-inflammatory activity in an inflamed intestinal mucosa model. To increase the accumulation of these two compounds in chicory taproots, the lactucin synthase that takes 8-deoxylactucin as the substrate for the regiospecific hydroxylation to generate lactucin needs to be inactivated. Three candidate cytochrome P450 enzymes of the CYP71 clan were identified in chicory. Their t2023-01-01T00:00:00Z2023 Apr2026-05-28T14:00:32.845Z2025-02-19T04:16:26.448ZS-EPMC101199873703679910.1021/acs.jafc.2c08959