biostudies-literatureUnknown21(1)Yadav Y<h4>Background</h4>PP1γ is one of the isoforms of catalytic subunit of a Ser/Thr phosphatase PP1. The role of PP1γ in cellular regulation is largely unknown. The present study investigated the role of PP1γ in regulating neuronal insulin signaling and insulin resistance in neuronal cells. PP1 was inhibited in mouse neuroblastoma cells (N2a) and human neuroblastoma cells (SH-SY5Y). The expression of PP1α and PP1γ was determined in insulin resistant N2a, SH-SY5Y cells and in high-fat-diet-fed-diabetic mice whole-brain-lysates. PP1α and PP1γ were silenced by siRNA in N2a and SH-SY5Y cells and effect was tested on AKT isoforms, AS160 and GSK3 isoforms using western immunoblot, GLUT4 translocation by confocal microscopy and glucose uptake by fluorescence-based assay.<h4>Results</h4>Results showeCell communication and signaling : CCS82https://www.ebi.ac.uk/biostudies/studies/S-EPMC10120118biostudies-literaturePP1γ regulates neuronal insulin signaling and aggravates insulin resistance leading to AD-like phenotypes.PMC10120118Yadav YDey CSSharma MfalsePP1γ regulates neuronal insulin signaling and aggravates insulin resistance leading to AD-like phenotypes.<h4>Background</h4>PP1γ is one of the isoforms of catalytic subunit of a Ser/Thr phosphatase PP1. The role of PP1γ in cellular regulation is largely unknown. The present study investigated the role of PP1γ in regulating neuronal insulin signaling and insulin resistance in neuronal cells. PP1 was inhibited in mouse neuroblastoma cells (N2a) and human neuroblastoma cells (SH-SY5Y). The expression of PP1α and PP1γ was determined in insulin resistant N2a, SH-SY5Y cells and in high-fat-diet-fed-diabetic mice whole-brain-lysates. PP1α and PP1γ were silenced by siRNA in N2a and SH-SY5Y cells and effect was tested on AKT isoforms, AS160 and GSK3 isoforms using western immunoblot, GLUT4 translocation by confocal microscopy and glucose uptake by fluorescence-based assay.<h4>Results</h4>Results showe2023-01-01T00:00:00Z2023 Apr2025-04-22T12:02:28.906Z2025-02-19T02:16:33.74ZS-EPMC101201183708581510.1186/s12964-023-01071-x