biostudies-literatureUnknown13(3)Bian XTrace amines (TAs) are metabolically related to catecholamine and associated with cancer and neurological disorders. Comprehensive measurement of TAs is essential for understanding pathological processes and providing proper drug intervention. However, the trace amounts and chemical instability of TAs challenge quantification. Here, diisopropyl phosphite coupled with chip two-dimensional (2D) liquid chromatography tandem triple-quadrupole mass spectrometry (LC-QQQ/MS) was developed to simultaneously determine TAs and associated metabolites. The results showed that the sensitivities of TAs increased up to 5520 times compared with those using nonderivatized LC-QQQ/MS. This sensitive method was utilized to investigate their alterations in hepatoma cells after treatment with sorafenib. The sigJournal of pharmaceutical analysis315-322https://www.ebi.ac.uk/biostudies/studies/S-EPMC10123937biostudies-literatureUltrasensitive quantification of trace amines based on <i>N</i>-phosphorylation labeling chip 2D LC-QQQ/MS.PMC10123937Liu JShi MZhang YBian XChen XWu JLZhang HLLi NfalseUltrasensitive quantification of trace amines based on <i>N</i>-phosphorylation labeling chip 2D LC-QQQ/MS.Trace amines (TAs) are metabolically related to catecholamine and associated with cancer and neurological disorders. Comprehensive measurement of TAs is essential for understanding pathological processes and providing proper drug intervention. However, the trace amounts and chemical instability of TAs challenge quantification. Here, diisopropyl phosphite coupled with chip two-dimensional (2D) liquid chromatography tandem triple-quadrupole mass spectrometry (LC-QQQ/MS) was developed to simultaneously determine TAs and associated metabolites. The results showed that the sensitivities of TAs increased up to 5520 times compared with those using nonderivatized LC-QQQ/MS. This sensitive method was utilized to investigate their alterations in hepatoma cells after treatment with sorafenib. The sig2023-01-01T00:00:00Z2023 Mar2025-04-18T16:29:16.767Z2025-02-19T04:15:48.594ZS-EPMC101239373710210710.1016/j.jpha.2023.02.003