biostudies-literatureWang SCNational Science and Technology Council, TaiwanAcademia Sinicae0017723https://www.ebi.ac.uk/biostudies/studies/S-EPMC10128044biostudies-literatureUnknown14(2)The type VI secretion system (T6SS) is deployed by many proteobacteria to secrete effector proteins into bacterial competitors for competition or eukaryotic cells for pathogenesis. Agrobacteria, a group of soilborne phytopathogens causing crown gall disease on various plant species, deploy the T6SS to attack closely and distantly related bacterial species <i>in vitro</i> and <i>in planta</i>. Current evidence suggests that the T6SS is not essential for pathogenesis under direct inoculation, but it remains unknown whether the T6SS influences natural disease incidence or the microbial community within crown galls (i.e., the gallobiome). To address these two key questions, we established a soil inoculation method on wounded tomato seedlings that mimics natural infections and developed a bactemBioSoil Inoculation and Blocker-Mediated Sequencing Show Effects of the Antibacterial T6SS on Agrobacterial Tumorigenesis and Gallobiome.PMC10128044111-2628-B-001-019AS-IA-107-L01110-2628-B-001-020109-2628-B-001-012Lai EMChou SJChen APWang SCKuo CHfalseSoil Inoculation and Blocker-Mediated Sequencing Show Effects of the Antibacterial T6SS on Agrobacterial Tumorigenesis and Gallobiome.The type VI secretion system (T6SS) is deployed by many proteobacteria to secrete effector proteins into bacterial competitors for competition or eukaryotic cells for pathogenesis. Agrobacteria, a group of soilborne phytopathogens causing crown gall disease on various plant species, deploy the T6SS to attack closely and distantly related bacterial species <i>in vitro</i> and <i>in planta</i>. Current evidence suggests that the T6SS is not essential for pathogenesis under direct inoculation, but it remains unknown whether the T6SS influences natural disease incidence or the microbial community within crown galls (i.e., the gallobiome). To address these two key questions, we established a soil inoculation method on wounded tomato seedlings that mimics natural infections and developed a bacte2023-01-01T00:00:00Z2023 Apr2026-06-13T06:16:16.738Z2024-12-04T10:46:32.995ZS-EPMC101280443687705410.1128/mbio.00177-23