{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["22(4)"],"submitter":["Palmieri M"],"funding":["National Health and Medical Research Council"],"pubmed_abstract":["The canonical view of PI3Kα signaling describes phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P<sub>3</sub>) generation and activation of downstream effectors at the plasma membrane or at microtubule-bound endosomes. Here, we show that colorectal cancer (CRC) cell lines exhibit a diverse plasma membrane-nuclear distribution of PI3Kα, controlling corresponding levels of subcellular PtdIns(3,4,5)P<sub>3</sub> pools. PI3Kα nuclear translocation was mediated by the importin β-dependent nuclear import pathway. By PtdIns(3,4,5)P<sub>3</sub> affinity capture mass spectrometry done in the presence of SDS on CRC cell lines with PI3Kα nuclear localization, we identified 867 potential nuclear PtdIns(3,4,5)P<sub>3</sub> effector proteins. Nuclear PtdIns(3,4,5)P<sub>3</sub> interactome protein"],"journal":["Molecular & cellular proteomics : MCP"],"pagination":["100529"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10130476"],"repository":["biostudies-literature"],"pubmed_title":["PI3Kα Translocation Mediates Nuclear PtdIns(3,4,5)P<sub>3</sub> Effector Signaling in Colorectal Cancer."],"pmcid":["PMC10130476"],"pubmed_authors":["Sieber OM","Mouradov D","Nowell CJ","Desai J","Catimel B","Palmieri M","Christie M","Sakthianandeswaren A","Williamson NA","Burgess AW","Gibbs P","Kapp E","Ang CS"],"additional_accession":[]},"is_claimable":false,"name":"PI3Kα Translocation Mediates Nuclear PtdIns(3,4,5)P<sub>3</sub> Effector Signaling in Colorectal Cancer.","description":"The canonical view of PI3Kα signaling describes phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P<sub>3</sub>) generation and activation of downstream effectors at the plasma membrane or at microtubule-bound endosomes. Here, we show that colorectal cancer (CRC) cell lines exhibit a diverse plasma membrane-nuclear distribution of PI3Kα, controlling corresponding levels of subcellular PtdIns(3,4,5)P<sub>3</sub> pools. PI3Kα nuclear translocation was mediated by the importin β-dependent nuclear import pathway. By PtdIns(3,4,5)P<sub>3</sub> affinity capture mass spectrometry done in the presence of SDS on CRC cell lines with PI3Kα nuclear localization, we identified 867 potential nuclear PtdIns(3,4,5)P<sub>3</sub> effector proteins. Nuclear PtdIns(3,4,5)P<sub>3</sub> interactome protein","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2026-07-14T21:05:15.349Z","creation":"2025-04-04T20:20:14.159Z"},"accession":"S-EPMC10130476","cross_references":{"pubmed":["36931626"],"doi":["10.1016/j.mcpro.2023.100529"]}}