biostudies-literatureUnknown22(4)Palmieri MNational Health and Medical Research CouncilThe canonical view of PI3Kα signaling describes phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P₃) generation and activation of downstream effectors at the plasma membrane or at microtubule-bound endosomes. Here, we show that colorectal cancer (CRC) cell lines exhibit a diverse plasma membrane-nuclear distribution of PI3Kα, controlling corresponding levels of subcellular PtdIns(3,4,5)P₃ pools. PI3Kα nuclear translocation was mediated by the importin β-dependent nuclear import pathway. By PtdIns(3,4,5)P₃ affinity capture mass spectrometry done in the presence of SDS on CRC cell lines with PI3Kα nuclear localization, we identified 867 potential nuclear PtdIns(3,4,5)P₃ effector proteins. Nuclear PtdIns(3,4,5)P₃ interactome proteinMolecular & cellular proteomics : MCP100529https://www.ebi.ac.uk/biostudies/studies/S-EPMC10130476biostudies-literaturePI3Kα Translocation Mediates Nuclear PtdIns(3,4,5)P₃ Effector Signaling in Colorectal Cancer.PMC10130476Sieber OMMouradov DNowell CJDesai JCatimel BPalmieri MChristie MSakthianandeswaren AWilliamson NABurgess AWGibbs PKapp EAng CSfalsePI3Kα Translocation Mediates Nuclear PtdIns(3,4,5)P₃ Effector Signaling in Colorectal Cancer.The canonical view of PI3Kα signaling describes phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P₃) generation and activation of downstream effectors at the plasma membrane or at microtubule-bound endosomes. Here, we show that colorectal cancer (CRC) cell lines exhibit a diverse plasma membrane-nuclear distribution of PI3Kα, controlling corresponding levels of subcellular PtdIns(3,4,5)P₃ pools. PI3Kα nuclear translocation was mediated by the importin β-dependent nuclear import pathway. By PtdIns(3,4,5)P₃ affinity capture mass spectrometry done in the presence of SDS on CRC cell lines with PI3Kα nuclear localization, we identified 867 potential nuclear PtdIns(3,4,5)P₃ effector proteins. Nuclear PtdIns(3,4,5)P₃ interactome protein2023-01-01T00:00:00Z2023 Apr2026-07-14T21:05:15.349Z2025-04-04T20:20:14.159ZS-EPMC101304763693162610.1016/j.mcpro.2023.100529