{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Terhorst A"],"funding":["Swiss National Science Foundation","Deutsche Forschungsgemeinschaft","NCI NIH HHS","NINDS NIH HHS","National Institutes of Health","NIGMS NIH HHS"],"pagination":["1118766"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10130656"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11"],"pubmed_abstract":["Prolonged cell cycle arrests occur naturally in differentiated cells and in response to various stresses such as nutrient deprivation or treatment with chemotherapeutic agents. Whether and how cells survive prolonged cell cycle arrests is not clear. Here, we used <i>S. cerevisiae</i> to compare physiological cell cycle arrests and genetically induced arrests in G1-, meta- and anaphase. Prolonged cell cycle arrest led to growth attenuation in all studied conditions, coincided with activation of the Environmental Stress Response (ESR) and with a reduced ribosome content as determined by whole ribosome purification and TMT mass spectrometry. Suppression of the ESR through hyperactivation of the Ras/PKA pathway reduced cell viability during prolonged arrests, demonstrating a cytoprotective role of the ESR. Attenuation of cell growth and activation of stress induced signaling pathways also occur in arrested human cell lines, raising the possibility that the response to prolonged cell cycle arrest is conserved."],"journal":["Frontiers in cell and developmental biology"],"pubmed_title":["The environmental stress response regulates ribosome content in cell cycle-arrested <i>S. cerevisiae</i>."],"pmcid":["PMC10130656"],"funding_grant_id":["417630603","R01 GM132447","R37 CA240765","R01 NS127186","P30 CA014051","CA206157 GM118066 GM132447 CA240765","R35 GM118066","R01 CA206157","PCEFP3_187003"],"pubmed_authors":["Sandikci A","Terhorst A","Szoradi T","Amon A","Whittaker CA","Holt LJ","Neurohr GE"],"additional_accession":[]},"is_claimable":false,"name":"The environmental stress response regulates ribosome content in cell cycle-arrested <i>S. cerevisiae</i>.","description":"Prolonged cell cycle arrests occur naturally in differentiated cells and in response to various stresses such as nutrient deprivation or treatment with chemotherapeutic agents. Whether and how cells survive prolonged cell cycle arrests is not clear. Here, we used <i>S. cerevisiae</i> to compare physiological cell cycle arrests and genetically induced arrests in G1-, meta- and anaphase. Prolonged cell cycle arrest led to growth attenuation in all studied conditions, coincided with activation of the Environmental Stress Response (ESR) and with a reduced ribosome content as determined by whole ribosome purification and TMT mass spectrometry. Suppression of the ESR through hyperactivation of the Ras/PKA pathway reduced cell viability during prolonged arrests, demonstrating a cytoprotective role of the ESR. Attenuation of cell growth and activation of stress induced signaling pathways also occur in arrested human cell lines, raising the possibility that the response to prolonged cell cycle arrest is conserved.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2025-06-25T03:03:59.553Z","creation":"2025-06-25T03:03:59.553Z"},"accession":"S-EPMC10130656","cross_references":{"pubmed":["37123399"],"doi":["10.3389/fcell.2023.1118766"]}}