biostudies-literatureCorrea RONIDDK NIH HHSMedical Research CouncilBiotechnology and Biological Sciences Research CouncilAcademy of Medical Sciences90https://www.ebi.ac.uk/biostudies/studies/S-EPMC10131329biostudies-literatureUnknown11(1)<h4>Background</h4>The continuous proliferation of intestinal stem cells followed by their tightly regulated differentiation to epithelial cells is essential for the maintenance of the gut epithelial barrier and its functions. How these processes are tuned by diet and gut microbiome is an important, but poorly understood question. Dietary soluble fibers, such as inulin, are known for their ability to impact the gut bacterial community and gut epithelium, and their consumption has been usually associated with health improvement in mice and humans. In this study, we tested the hypothesis that inulin consumption modifies the composition of colonic bacteria and this impacts intestinal stem cells functions, thus affecting the epithelial structure.<h4>Methods</h4>Mice were fed with a diet contaiMicrobiomeInulin diet uncovers complex diet-microbiota-immune cell interactions remodeling the gut epithelium.PMC10131329G0701175R01 DK126969BB/L026988/1GCRFNGR3\1130MR/N009398/1BB/N013565/1Imada SCorrea ROMatheus VAAraujo NVPColonna Mde Oliveira SRMDos Santos Martins Fde Rezende Rodovalho VNirello VDFachi JLPereira GVde Souza Felipe JOliveira SCPral LPCastro PRDos Santos Pereira Gomes ABVarga-Weisz Pde Oliveira SMartens EEl-Sahhar SVinolo MARFernandes MFde Assis HCfalseInulin diet uncovers complex diet-microbiota-immune cell interactions remodeling the gut epithelium.<h4>Background</h4>The continuous proliferation of intestinal stem cells followed by their tightly regulated differentiation to epithelial cells is essential for the maintenance of the gut epithelial barrier and its functions. How these processes are tuned by diet and gut microbiome is an important, but poorly understood question. Dietary soluble fibers, such as inulin, are known for their ability to impact the gut bacterial community and gut epithelium, and their consumption has been usually associated with health improvement in mice and humans. In this study, we tested the hypothesis that inulin consumption modifies the composition of colonic bacteria and this impacts intestinal stem cells functions, thus affecting the epithelial structure.<h4>Methods</h4>Mice were fed with a diet contai2023-01-01T00:00:00Z2023 Apr2026-07-14T21:05:17.437Z2025-02-19T02:17:08.606ZS-EPMC101313293710120910.1186/s40168-023-01520-2