biostudies-literatureUnknown9(3)Dusadeepong RIn early 2020, the Medical Biology Laboratory of the Pasteur Institute of Cambodia isolated an unusually high number of fluoroquinolone-resistant <i>Salmonella enterica</i> subspecies <i>enterica</i> serovar Paratyphi A strains during its routine bacteriological surveillance activities in Phnom Penh, Cambodia. A public-health investigation was supported by genome sequencing of these Paratyphi A strains to gain insights into the genetic diversity and population structure of a potential outbreak of fluoroquinolone-resistant paratyphoid fever. Comparative genomic and phylodynamic analyses revealed the 2020 strains were descended from a previously described 2013-2015 outbreak of Paratyphi A infections. Our analysis showed sub-lineage 2.3.1 had remained largely susceptible to fluoroquinolone drMicrobial genomicshttps://www.ebi.ac.uk/biostudies/studies/S-EPMC10132074biostudies-literaturePhylogenomic investigation of an outbreak of fluoroquinolone-resistant <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Paratyphi A in Phnom Penh, Cambodia.PMC10132074Choun KMeng SHardy LGlaser PVandelannoote KDusadeepong RTeav SCheng SHoang TJacobs JStinear TPLetchford JHowden BPSreng NSeemann TDelvallez GfalsePhylogenomic investigation of an outbreak of fluoroquinolone-resistant <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Paratyphi A in Phnom Penh, Cambodia.In early 2020, the Medical Biology Laboratory of the Pasteur Institute of Cambodia isolated an unusually high number of fluoroquinolone-resistant <i>Salmonella enterica</i> subspecies <i>enterica</i> serovar Paratyphi A strains during its routine bacteriological surveillance activities in Phnom Penh, Cambodia. A public-health investigation was supported by genome sequencing of these Paratyphi A strains to gain insights into the genetic diversity and population structure of a potential outbreak of fluoroquinolone-resistant paratyphoid fever. Comparative genomic and phylodynamic analyses revealed the 2020 strains were descended from a previously described 2013-2015 outbreak of Paratyphi A infections. Our analysis showed sub-lineage 2.3.1 had remained largely susceptible to fluoroquinolone dr2023-01-01T00:00:00Z2023 Mar2026-07-14T21:07:59.546Z2025-02-19T02:04:05.202ZS-EPMC101320743696148410.1099/mgen.0.000972