{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang ZL"],"funding":["National Natural Science Foundation of China","National Postdoctoral Program for Innovative Talents"],"pagination":["4418-4425"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10132108"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(16)"],"pubmed_abstract":["A highly efficient and promiscuous 7,4'-di-<i>O</i>-glycosyltransferase ZjOGT3 was discovered from the medicinal plant <i>Ziziphus jujuba</i> var. <i>spinosa</i>. ZjOGT3 could sequentially catalyse 4'- and 7-<i>O</i>-glycosylation of flavones to produce 7,4'-di-<i>O</i>-glycosides with obvious regio-selectivity. For 7,4'-dihydroxyl flavanones and 3-<i>O</i>-glycosylated 7,4'-dihydroxyl flavones, ZjOGT3 selectively catalyses 7-<i>O</i>-glycosylation. The crystal structure of ZjOGT3 was solved. Structural analysis, DFT calculations, MD simulations, and site-directed mutagenesis reveal that the regio-selectivity is mainly controlled by the enzyme microenvironment for 7,4'-dihydroxyl flavones and 3-<i>O</i>-glycosylated 7,4'-dihydroxyl flavones. For 7,4'-dihydroxyl flavanones, the selectivity "],"journal":["Chemical science"],"pubmed_title":["Functional characterization, structural basis, and regio-selectivity control of a promiscuous flavonoid 7,4'-di-<i>O</i>-glycosyltransferase from <i>Ziziphus jujuba</i> var. <i>spinosa</i>."],"pmcid":["PMC10132108"],"funding_grant_id":["BX20220022","81725023"],"pubmed_authors":["Ye M","Qiao X","Chen K","Zhou JJ","Liang Y","Wang HD","Wang HT","Wei W","Zhou H","Wang ZL","Li FD","Wang RS"],"additional_accession":[]},"is_claimable":false,"name":"Functional characterization, structural basis, and regio-selectivity control of a promiscuous flavonoid 7,4'-di-<i>O</i>-glycosyltransferase from <i>Ziziphus jujuba</i> var. <i>spinosa</i>.","description":"A highly efficient and promiscuous 7,4'-di-<i>O</i>-glycosyltransferase ZjOGT3 was discovered from the medicinal plant <i>Ziziphus jujuba</i> var. <i>spinosa</i>. ZjOGT3 could sequentially catalyse 4'- and 7-<i>O</i>-glycosylation of flavones to produce 7,4'-di-<i>O</i>-glycosides with obvious regio-selectivity. For 7,4'-dihydroxyl flavanones and 3-<i>O</i>-glycosylated 7,4'-dihydroxyl flavones, ZjOGT3 selectively catalyses 7-<i>O</i>-glycosylation. The crystal structure of ZjOGT3 was solved. Structural analysis, DFT calculations, MD simulations, and site-directed mutagenesis reveal that the regio-selectivity is mainly controlled by the enzyme microenvironment for 7,4'-dihydroxyl flavones and 3-<i>O</i>-glycosylated 7,4'-dihydroxyl flavones. For 7,4'-dihydroxyl flavanones, the selectivity ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2026-05-29T06:37:27.456Z","creation":"2024-11-13T07:31:38.29Z"},"accession":"S-EPMC10132108","cross_references":{"pubmed":["37123177"],"doi":["10.1039/d2sc06504e"]}}