{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Droho S"],"funding":["National Institute of Arthritis and Musculoskeletal and Skin Diseases","NIAID NIH HHS","Rheumatology Research Foundation","National Heart, Lung, and Blood Institute","Northwestern University","Lupus Research Alliance","National Institute on Aging","United States-Israel Binational Science Foundation","NEI NIH HHS","National Eye Institute","Research to Prevent Blindness","National Cancer Institute","NCI NIH HHS","NIAMS NIH HHS"],"pagination":["e168142"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10132149"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(7)"],"pubmed_abstract":["Patients with neovascular AMD (nAMD) suffer vision loss from destructive angiogenesis, termed choroidal neovascularization (CNV). Macrophages are found in CNV lesions from patients with nAMD. Additionally, Ccr2-/- mice, which lack classical monocyte-derived macrophages, show reduced CNV size. However, macrophages are highly diverse cells that can perform multiple functions. We performed single-cell RNA-Seq on immune cells from WT and Ccr2-/- eyes to uncover macrophage heterogeneity during the laser-induced CNV mouse model of nAMD. We identified 12 macrophage clusters, including Spp1+ macrophages. Spp1+ macrophages were enriched from WT lasered eyes and expressed a proangiogenic transcriptome via multiple pathways, including vascular endothelial growth factor signaling, endothelial cell spr"],"journal":["JCI insight"],"pubmed_title":["CD11c+ macrophages are proangiogenic and necessary for experimental choroidal neovascularization."],"pmcid":["PMC10132149"],"funding_grant_id":["AG049665","EY030923","Unrestricted Departmental Grant","UH2 AR067687","UH2AR067687","HL134375","Agmt 05/06/14","Rheumatology Precision Medicine Fund","Dixon Translational Research Grant Initiative Award","Novel Research Grant","Innovative Research Award","P30 CA060553","Sybil B. Harrington Career Development Award for Macular Degeneration","R01 AI170938","K08 EY030923","CA060553","AR064546","2013247"],"pubmed_authors":["Rajesh A","Lavine JA","Droho S","Perlman H","Cuda CM"],"additional_accession":[]},"is_claimable":false,"name":"CD11c+ macrophages are proangiogenic and necessary for experimental choroidal neovascularization.","description":"Patients with neovascular AMD (nAMD) suffer vision loss from destructive angiogenesis, termed choroidal neovascularization (CNV). Macrophages are found in CNV lesions from patients with nAMD. Additionally, Ccr2-/- mice, which lack classical monocyte-derived macrophages, show reduced CNV size. However, macrophages are highly diverse cells that can perform multiple functions. We performed single-cell RNA-Seq on immune cells from WT and Ccr2-/- eyes to uncover macrophage heterogeneity during the laser-induced CNV mouse model of nAMD. We identified 12 macrophage clusters, including Spp1+ macrophages. Spp1+ macrophages were enriched from WT lasered eyes and expressed a proangiogenic transcriptome via multiple pathways, including vascular endothelial growth factor signaling, endothelial cell spr","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2026-05-29T06:36:15.481Z","creation":"2025-02-19T02:03:57.06Z"},"accession":"S-EPMC10132149","cross_references":{"pubmed":["36821388"],"doi":["10.1172/jci.insight.168142"]}}