biostudies-literaturePremadasa LSNIDA NIH HHSNIAID NIH HHSNIMH NIH HHSNational Institute on Drug AbuseNIH HHSe167903https://www.ebi.ac.uk/biostudies/studies/S-EPMC10132162biostudies-literatureUnknown8(7)Intestinal epithelial barrier dysfunction, a hallmark of HIV/SIV infection, persists despite viral suppression by combination antiretroviral therapy (cART). Emerging evidence suggests a critical role for long noncoding RNAs (lncRNAs) in maintaining epithelial homeostasis. We simultaneously profiled lncRNA/mRNA expression exclusively in colonic epithelium (CE) of SIV-infected rhesus macaques (RMs) administered vehicle (VEH) or Δ-9-tetrahydrocannabinol (THC). Relative to controls, fewer lncRNAs were up- or downregulated in CE of THC/SIV compared with VEH/SIV RMs. Importantly, reciprocal expression of the natural antisense lncRNA MMP25-AS1 (up 2.3-fold) and its associated protein-coding gene MMP25 (attracts neutrophils by inactivating alpha-1 anti-trypsin/SERPINA1) (down 2.2-fold) was detecteJCI insightCannabinoid enhancement of lncRNA MMP25-AS1/MMP25 interaction reduces neutrophil infiltration and intestinal epithelial injury in HIV/SIV infection.PMC10132162P30 AI161943P51 OD011104R01 MH116844R01 DA052845R33 DA053643P51 OD011133R01DA042524,R01DA052845,R01DA050169,P30AI161943,P51OD011104,P51OD111033R01 DA050169R01 MH130193U42 OD010442R56 AI150371R01 AI157850R01 DA042524Jayakumar SMohan MAlvarez XLing BMcDew-White MPremadasa LSOkeoma CMByrareddy SNKulkarni SLee EfalseCannabinoid enhancement of lncRNA MMP25-AS1/MMP25 interaction reduces neutrophil infiltration and intestinal epithelial injury in HIV/SIV infection.Intestinal epithelial barrier dysfunction, a hallmark of HIV/SIV infection, persists despite viral suppression by combination antiretroviral therapy (cART). Emerging evidence suggests a critical role for long noncoding RNAs (lncRNAs) in maintaining epithelial homeostasis. We simultaneously profiled lncRNA/mRNA expression exclusively in colonic epithelium (CE) of SIV-infected rhesus macaques (RMs) administered vehicle (VEH) or Δ-9-tetrahydrocannabinol (THC). Relative to controls, fewer lncRNAs were up- or downregulated in CE of THC/SIV compared with VEH/SIV RMs. Importantly, reciprocal expression of the natural antisense lncRNA MMP25-AS1 (up 2.3-fold) and its associated protein-coding gene MMP25 (attracts neutrophils by inactivating alpha-1 anti-trypsin/SERPINA1) (down 2.2-fold) was detecte2023-01-01T00:00:00Z2023 Apr2026-05-29T06:37:33.804Z2025-02-19T02:04:05.813ZS-EPMC101321623703600710.1172/jci.insight.167903