{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["616(7958)"],"submitter":["Pradhan B"],"pubmed_abstract":["Structural maintenance of chromosomes (SMC) protein complexes are essential for the spatial organization of chromosomes<sup>1</sup>. Whereas cohesin and condensin organize chromosomes by extrusion of DNA loops, the molecular functions of the third eukaryotic SMC complex, Smc5/6, remain largely unknown<sup>2</sup>. Using single-molecule imaging, we show that Smc5/6 forms DNA loops by extrusion. Upon ATP hydrolysis, Smc5/6 reels DNA symmetrically into loops at a force-dependent rate of one kilobase pair per second. Smc5/6 extrudes loops in the form of dimers, whereas monomeric Smc5/6 unidirectionally translocates along DNA. We also find that the subunits Nse5 and Nse6 (Nse5/6) act as negative regulators of loop extrusion. Nse5/6 inhibits loop-extrusion initiation by hindering Smc5/6 dimeriza"],"journal":["Nature"],"pagination":["843-848"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10132971"],"repository":["biostudies-literature"],"pubmed_title":["The Smc5/6 complex is a DNA loop-extruding motor."],"pmcid":["PMC10132971"],"pubmed_authors":["Bjorkegren C","Pradhan B","Kanno T","Kim E","Umeda Igarashi M","Wong JSK","Jeppsson K","Loke MS","Baaske MD"],"additional_accession":[]},"is_claimable":false,"name":"The Smc5/6 complex is a DNA loop-extruding motor.","description":"Structural maintenance of chromosomes (SMC) protein complexes are essential for the spatial organization of chromosomes<sup>1</sup>. Whereas cohesin and condensin organize chromosomes by extrusion of DNA loops, the molecular functions of the third eukaryotic SMC complex, Smc5/6, remain largely unknown<sup>2</sup>. Using single-molecule imaging, we show that Smc5/6 forms DNA loops by extrusion. Upon ATP hydrolysis, Smc5/6 reels DNA symmetrically into loops at a force-dependent rate of one kilobase pair per second. Smc5/6 extrudes loops in the form of dimers, whereas monomeric Smc5/6 unidirectionally translocates along DNA. We also find that the subunits Nse5 and Nse6 (Nse5/6) act as negative regulators of loop extrusion. Nse5/6 inhibits loop-extrusion initiation by hindering Smc5/6 dimeriza","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2025-04-22T10:05:09.582Z","creation":"2025-04-05T23:23:23.026Z"},"accession":"S-EPMC10132971","cross_references":{"pubmed":["37076626"],"doi":["10.1038/s41586-023-05963-3"]}}