biostudies-literatureUnknown12(4)Scandorieiro SResistant bacteria may kill more people than COVID-19, so the development of new antibacterials is essential, especially against microbial biofilms that are reservoirs of resistant cells. Silver nanoparticles (bioAgNP), biogenically synthesized using <i>Fusarium oxysporum</i>, combined with oregano derivatives, present a strategic antibacterial mechanism and prevent the emergence of resistance against planktonic microorganisms. Antibiofilm activity of four binary combinations was tested against enteroaggregative <i>Escherichia coli</i> (EAEC) and <i>Klebsiella pneumoniae</i> carbapenemase-producing <i>K. pneumoniae</i> (KPC): oregano essential oil (OEO) plus bioAgNP, carvacrol (Car) plus bioAgNP, thymol (Thy) plus bioAgNP, and Car plus Thy. The antibiofilm effect was accessed using crystalAntibiotics (Basel, Switzerland)756https://www.ebi.ac.uk/biostudies/studies/S-EPMC10135348biostudies-literatureAntibiofilm Effect of Biogenic Silver Nanoparticles Combined with Oregano Derivatives against Carbapenem-Resistant <i>Klebsiella pneumoniae</i>.PMC10135348Panagio LATeixeira FMMBde Oliveira AGKobayashi RKTNakazato GNogueira MCLScandorieiro SDuran NfalseAntibiofilm Effect of Biogenic Silver Nanoparticles Combined with Oregano Derivatives against Carbapenem-Resistant <i>Klebsiella pneumoniae</i>.Resistant bacteria may kill more people than COVID-19, so the development of new antibacterials is essential, especially against microbial biofilms that are reservoirs of resistant cells. Silver nanoparticles (bioAgNP), biogenically synthesized using <i>Fusarium oxysporum</i>, combined with oregano derivatives, present a strategic antibacterial mechanism and prevent the emergence of resistance against planktonic microorganisms. Antibiofilm activity of four binary combinations was tested against enteroaggregative <i>Escherichia coli</i> (EAEC) and <i>Klebsiella pneumoniae</i> carbapenemase-producing <i>K. pneumoniae</i> (KPC): oregano essential oil (OEO) plus bioAgNP, carvacrol (Car) plus bioAgNP, thymol (Thy) plus bioAgNP, and Car plus Thy. The antibiofilm effect was accessed using crystal2023-01-01T00:00:00Z2023 Apr2025-04-04T11:05:31.802Z2025-04-04T11:05:31.802ZS-EPMC101353483710711910.3390/antibiotics12040756