biostudies-literatureOsipov AVVietnamese Academy of Sciences and TechnologyRussian Foundation for Basic Research1115https://www.ebi.ac.uk/biostudies/studies/S-EPMC10136136biostudies-literatureUnknown11(4)Protozoal infections are a world-wide problem. The toxicity and somewhat low effectiveness of the existing drugs require the search for new ways of protozoa suppression. Snake venom contains structurally diverse components manifesting antiprotozoal activity; for example, those in cobra venom are cytotoxins. In this work, we aimed to characterize a novel antiprotozoal component(s) in the <i>Bungarus multicinctus</i> krait venom using the ciliate <i>Tetrahymena pyriformis</i> as a model organism. To determine the toxicity of the substances under study, surviving ciliates were registered automatically by an original BioLaT-3.2 instrument. The krait venom was separated by three-step liquid chromatography and the toxicity of the obtained fractions against <i>T. pyriformis</i> was analyzed. As aBiomedicinesThe Potassium Channel Blocker β-Bungarotoxin from the Krait <i>Bungarus multicinctus</i> Venom Manifests Antiprotozoal Activity.PMC1013613621-54-54005QTRU01.15/21-22Cheremnykh EGHoang ANStarkov VGUtkin YNNguyen TTTTsetlin VIOsipov AVNguyen KCLe DTZiganshin RHfalseThe Potassium Channel Blocker β-Bungarotoxin from the Krait <i>Bungarus multicinctus</i> Venom Manifests Antiprotozoal Activity.Protozoal infections are a world-wide problem. The toxicity and somewhat low effectiveness of the existing drugs require the search for new ways of protozoa suppression. Snake venom contains structurally diverse components manifesting antiprotozoal activity; for example, those in cobra venom are cytotoxins. In this work, we aimed to characterize a novel antiprotozoal component(s) in the <i>Bungarus multicinctus</i> krait venom using the ciliate <i>Tetrahymena pyriformis</i> as a model organism. To determine the toxicity of the substances under study, surviving ciliates were registered automatically by an original BioLaT-3.2 instrument. The krait venom was separated by three-step liquid chromatography and the toxicity of the obtained fractions against <i>T. pyriformis</i> was analyzed. As a2023-01-01T00:00:00Z2023 Apr2025-04-22T05:17:02.18Z2025-04-05T21:16:51.698ZS-EPMC101361363718973310.3390/biomedicines11041115