biostudies-literatureDigomann DJung StiftungDeutsche ForschungsgemeinschaftElse Kröner-Fresenius-StiftungMonika Kutzner Stiftung2326https://www.ebi.ac.uk/biostudies/studies/S-EPMC10136841biostudies-literatureUnknown15(8)Immunotherapy has shown promising results in multiple solid tumors and hematological malignancies. However, pancreatic ductal adenocarcinoma (PDAC) has been largely refractory to current clinical immunotherapies. The V-domain Ig suppressor of T-cell activation (VISTA) inhibits T-cell effector function and maintains peripheral tolerance. Here, we determine VISTA expression in nontumorous pancreatic (<i>n</i> = 5) and PDAC tissue using immunohistochemistry (<i>n</i> = 76) and multiplex immunofluorescence staining (<i>n</i> = 67). Additionally, VISTA expression on tumor-infiltrating immune cells and matched blood samples (<i>n</i> = 13) was measured with multicolor flow cytometry. Further, the effect of recombinant VISTA on T-cell activation was investigated in vitro, and VISTA blockade was tCancersVISTA Ligation Reduces Antitumor T-Cell Activity in Pancreatic Cancer.PMC10136841SE2980/5-1NAStrack JDistler MPlesca IReiche CSeifert LBeer CSchmitz MDigomann DSeifert AMHeiduk MSommer URupp LNicolaus SWeitz JfalseVISTA Ligation Reduces Antitumor T-Cell Activity in Pancreatic Cancer.Immunotherapy has shown promising results in multiple solid tumors and hematological malignancies. However, pancreatic ductal adenocarcinoma (PDAC) has been largely refractory to current clinical immunotherapies. The V-domain Ig suppressor of T-cell activation (VISTA) inhibits T-cell effector function and maintains peripheral tolerance. Here, we determine VISTA expression in nontumorous pancreatic (<i>n</i> = 5) and PDAC tissue using immunohistochemistry (<i>n</i> = 76) and multiplex immunofluorescence staining (<i>n</i> = 67). Additionally, VISTA expression on tumor-infiltrating immune cells and matched blood samples (<i>n</i> = 13) was measured with multicolor flow cytometry. Further, the effect of recombinant VISTA on T-cell activation was investigated in vitro, and VISTA blockade was t2023-01-01T00:00:00Z2023 Apr2025-04-22T05:15:21.046Z2025-04-05T21:16:36.855ZS-EPMC101368413719025410.3390/cancers15082326