{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(8)"],"submitter":["Kato K"],"pubmed_abstract":["The recent increase in the number of molecular targeted agents for lung cancer has led to the demand for the simultaneous testing of multiple genes. Although gene panels using next-generation sequencing (NGS) are ideal, conventional panels require a high tumor content, and biopsy samples often do not meet this requirement. We developed a new NGS panel, called compact panel, characterized by high sensitivity, with detection limits for mutations of 0.14%, 0.20%, 0.48%, 0.24%, and 0.20% for <i>EGFR</i> exon 19 deletion, L858R, T790M, <i>BRAF</i> V600E, and <i>KRAS</i> G12C, respectively. Mutation detection also had a high quantitative ability, with correlation coefficients ranging from 0.966 to 0.992. The threshold for fusion detection was 1%. The panel exhibited good concordance with the app"],"journal":["Diagnostics (Basel, Switzerland)"],"pagination":["1476"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10137435"],"repository":["biostudies-literature"],"pubmed_title":["Analytical Performance of a Highly Sensitive System to Detect Gene Variants Using Next-Generation Sequencing for Lung Cancer Companion Diagnostics."],"pmcid":["PMC10137435"],"pubmed_authors":["Kato K","Nakamura H","Okami J","Nakatsuka SI","Kukita Y","Higashiyama M","Honma K","Sato Y","Nakamura S"],"additional_accession":[]},"is_claimable":false,"name":"Analytical Performance of a Highly Sensitive System to Detect Gene Variants Using Next-Generation Sequencing for Lung Cancer Companion Diagnostics.","description":"The recent increase in the number of molecular targeted agents for lung cancer has led to the demand for the simultaneous testing of multiple genes. Although gene panels using next-generation sequencing (NGS) are ideal, conventional panels require a high tumor content, and biopsy samples often do not meet this requirement. We developed a new NGS panel, called compact panel, characterized by high sensitivity, with detection limits for mutations of 0.14%, 0.20%, 0.48%, 0.24%, and 0.20% for <i>EGFR</i> exon 19 deletion, L858R, T790M, <i>BRAF</i> V600E, and <i>KRAS</i> G12C, respectively. Mutation detection also had a high quantitative ability, with correlation coefficients ranging from 0.966 to 0.992. The threshold for fusion detection was 1%. The panel exhibited good concordance with the app","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2025-04-05T21:14:43.85Z","creation":"2025-04-05T21:14:43.85Z"},"accession":"S-EPMC10137435","cross_references":{"pubmed":["37189577"],"doi":["10.3390/diagnostics13081476"]}}