biostudies-literatureUnknown13(8)Kato KThe recent increase in the number of molecular targeted agents for lung cancer has led to the demand for the simultaneous testing of multiple genes. Although gene panels using next-generation sequencing (NGS) are ideal, conventional panels require a high tumor content, and biopsy samples often do not meet this requirement. We developed a new NGS panel, called compact panel, characterized by high sensitivity, with detection limits for mutations of 0.14%, 0.20%, 0.48%, 0.24%, and 0.20% for <i>EGFR</i> exon 19 deletion, L858R, T790M, <i>BRAF</i> V600E, and <i>KRAS</i> G12C, respectively. Mutation detection also had a high quantitative ability, with correlation coefficients ranging from 0.966 to 0.992. The threshold for fusion detection was 1%. The panel exhibited good concordance with the appDiagnostics (Basel, Switzerland)1476https://www.ebi.ac.uk/biostudies/studies/S-EPMC10137435biostudies-literatureAnalytical Performance of a Highly Sensitive System to Detect Gene Variants Using Next-Generation Sequencing for Lung Cancer Companion Diagnostics.PMC10137435Kato KNakamura HOkami JNakatsuka SIKukita YHigashiyama MHonma KSato YNakamura SfalseAnalytical Performance of a Highly Sensitive System to Detect Gene Variants Using Next-Generation Sequencing for Lung Cancer Companion Diagnostics.The recent increase in the number of molecular targeted agents for lung cancer has led to the demand for the simultaneous testing of multiple genes. Although gene panels using next-generation sequencing (NGS) are ideal, conventional panels require a high tumor content, and biopsy samples often do not meet this requirement. We developed a new NGS panel, called compact panel, characterized by high sensitivity, with detection limits for mutations of 0.14%, 0.20%, 0.48%, 0.24%, and 0.20% for <i>EGFR</i> exon 19 deletion, L858R, T790M, <i>BRAF</i> V600E, and <i>KRAS</i> G12C, respectively. Mutation detection also had a high quantitative ability, with correlation coefficients ranging from 0.966 to 0.992. The threshold for fusion detection was 1%. The panel exhibited good concordance with the app2023-01-01T00:00:00Z2023 Apr2025-04-05T21:14:43.85Z2025-04-05T21:14:43.85ZS-EPMC101374353718957710.3390/diagnostics13081476