biostudies-literatureLoh JWMOH | National Medical Research CouncilMOH | National Medical Research Council (NMRC)461https://www.ebi.ac.uk/biostudies/studies/S-EPMC10140281biostudies-literatureUnknown6(1)Angiosarcomas are rare malignant tumors of the endothelium, arising commonly from the head and neck region (AS-HN) and recently associated with ultraviolet (UV) exposure and human herpesvirus-7 infection. We examined 81 cases of angiosarcomas, including 47 cases of AS-HN, integrating information from whole genome sequencing, gene expression profiling and spatial transcriptomics (10X Visium). In the AS-HN cohort, we observed recurrent somatic mutations in CSMD3 (18%), LRP1B (18%), MUC16 (18%), POT1 (16%) and TP53 (16%). UV-positive AS-HN harbored significantly higher tumor mutation burden than UV-negative cases (p = 0.0294). NanoString profiling identified three clusters with distinct tumor inflammation signature scores (p < 0.001). Spatial transcriptomics revealed topological profiles of tCommunications biologySpatial transcriptomics reveal topological immune landscapes of Asian head and neck angiosarcoma.PMC10140281NMRC/STAR/0006/2009TA21jun-0005CG21APR200SEEDFD21jun-0002Kannan BKo TKGu NXOng CKLee JYGuan PLim BYTeh BTLoh JWYeong JLee ECYNg CCLim JCTLim JQLim AHChan JYfalseSpatial transcriptomics reveal topological immune landscapes of Asian head and neck angiosarcoma.Angiosarcomas are rare malignant tumors of the endothelium, arising commonly from the head and neck region (AS-HN) and recently associated with ultraviolet (UV) exposure and human herpesvirus-7 infection. We examined 81 cases of angiosarcomas, including 47 cases of AS-HN, integrating information from whole genome sequencing, gene expression profiling and spatial transcriptomics (10X Visium). In the AS-HN cohort, we observed recurrent somatic mutations in CSMD3 (18%), LRP1B (18%), MUC16 (18%), POT1 (16%) and TP53 (16%). UV-positive AS-HN harbored significantly higher tumor mutation burden than UV-negative cases (p = 0.0294). NanoString profiling identified three clusters with distinct tumor inflammation signature scores (p < 0.001). Spatial transcriptomics revealed topological profiles of t2023-01-01T00:00:00Z2023 Apr2025-04-26T01:28:42.045Z2025-04-06T10:04:49.339ZS-EPMC101402813710602710.1038/s42003-023-04856-5