{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jia Z"],"funding":["Science and Technology Program of Hunan Province","National Natural Science Foundation of China"],"pagination":["1156219"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10140343"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14"],"pubmed_abstract":["<h4>Introduction</h4>Cartilaginous fishes are the most evolutionary-distant vertebrates from mammals and possess an immunoglobulin (Ig)- and T cell-mediated adaptive immunity. CD8 is the hallmark receptor of cytotoxic T cells and is required for the formation of T cell receptor-major histocompatibility complex (TCR-MHC) class I complexes.<h4>Methods</h4>RACE PCR was used to obtain gene sequences. Direct dilution was applied for the refolding of denatured recombinant CD8 protein. Hanging-drop vapor diffusion method was performed for protein crystallization.<h4>Results</h4>In this study, CD8α and CD8β orthologues (termed ScCD8α and ScCD8β) were identified in small-spotted catshark (<i>Scyliorhinus canicula</i>). Both ScCD8α and ScCD8β possess an extracellular immunoglobulin superfamily (IgSF"],"journal":["Frontiers in immunology"],"pubmed_title":["The first crystal structure of CD8αα from a cartilaginous fish."],"pmcid":["PMC10140343"],"funding_grant_id":["32030112 , U21A20268"],"pubmed_authors":["Zou J","Feng J","Dooley H","Wang J","Jia Z"],"additional_accession":[]},"is_claimable":false,"name":"The first crystal structure of CD8αα from a cartilaginous fish.","description":"<h4>Introduction</h4>Cartilaginous fishes are the most evolutionary-distant vertebrates from mammals and possess an immunoglobulin (Ig)- and T cell-mediated adaptive immunity. CD8 is the hallmark receptor of cytotoxic T cells and is required for the formation of T cell receptor-major histocompatibility complex (TCR-MHC) class I complexes.<h4>Methods</h4>RACE PCR was used to obtain gene sequences. Direct dilution was applied for the refolding of denatured recombinant CD8 protein. Hanging-drop vapor diffusion method was performed for protein crystallization.<h4>Results</h4>In this study, CD8α and CD8β orthologues (termed ScCD8α and ScCD8β) were identified in small-spotted catshark (<i>Scyliorhinus canicula</i>). Both ScCD8α and ScCD8β possess an extracellular immunoglobulin superfamily (IgSF","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-05-29T02:23:39.362Z","creation":"2025-02-19T02:23:59.56Z"},"accession":"S-EPMC10140343","cross_references":{"pubmed":["37122697"],"doi":["10.3389/fimmu.2023.1156219"]}}