biostudies-literatureSahin SINIH HHS736-749https://www.ebi.ac.uk/biostudies/studies/S-EPMC10143874biostudies-literatureUnknown9(2)Metabolite-specific echo-planar imaging (EPI) sequences with spectral-spatial (spsp) excitation are commonly used in clinical hyperpolarized [1-¹³C]pyruvate studies because of their speed, efficiency, and flexibility. In contrast, preclinical systems typically rely on slower spectroscopic methods, such as chemical shift imaging (CSI). In this study, a 2D spspEPI sequence was developed for use on a preclinical 3T Bruker system and tested on in vivo mice experiments with patient-derived xenograft renal cell carcinoma (RCC) or prostate cancer tissues implanted in the kidney or liver. Compared to spspEPI sequences, CSI were found to have a broader point spread function via simulations and exhibited signal bleeding between vasculature and tumors in vivo. Parameters for the spspEPI seTomography (Ann Arbor, Mich.)Metabolite-Specific Echo Planar Imaging for Preclinical Studies with Hyperpolarized ¹³C-Pyruvate MRI.PMC10143874R01CA249909P41EB013598U24CA253377Mattingly MSinha AGordon JWJi XMali IAgarwal SSahin SIKurhanewicz JSubramaniam SLarson PEZSriram RfalseMetabolite-Specific Echo Planar Imaging for Preclinical Studies with Hyperpolarized ¹³C-Pyruvate MRI.Metabolite-specific echo-planar imaging (EPI) sequences with spectral-spatial (spsp) excitation are commonly used in clinical hyperpolarized [1-¹³C]pyruvate studies because of their speed, efficiency, and flexibility. In contrast, preclinical systems typically rely on slower spectroscopic methods, such as chemical shift imaging (CSI). In this study, a 2D spspEPI sequence was developed for use on a preclinical 3T Bruker system and tested on in vivo mice experiments with patient-derived xenograft renal cell carcinoma (RCC) or prostate cancer tissues implanted in the kidney or liver. Compared to spspEPI sequences, CSI were found to have a broader point spread function via simulations and exhibited signal bleeding between vasculature and tumors in vivo. Parameters for the spspEPI se2023-01-01T00:00:00Z2023 Mar2025-04-18T16:29:57.604Z2025-04-07T03:43:29.302ZS-EPMC101438743710413010.3390/tomography9020059