biostudies-literatureYang LNational Natural Science Foundation of ChinaThe Postgraduate Innovative Research Projects of TianjinNatural Science Foundation of Tianjin1715https://www.ebi.ac.uk/biostudies/studies/S-EPMC10144588biostudies-literatureUnknown12(8)With the development of aquaculture, wastewater treatment and diseases have been paid more and more attention. The question of how to improve the immunity of aquatic species, as well as treat aquaculture wastewater, has become an urgent problem. In this study, duckweed with a high protein content (37.4%) (<i>Lemna turionifera</i> 5511) has been employed as a feedstock for aquatic wastewater treatment and the production of antimicrobial peptides. Penaeidins 3a (Pen3a), from <i>Litopenaeus vannamei</i>, were expressed under the control of CaMV-35S promoter in duckweed. Bacteriostatic testing using the Pen3a duckweed extract showed its antibacterial activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. Transcriptome analysis of wild type (WT) duckweed and Pen3a duckweed sPlants (Basel, Switzerland)The Antimicrobial Potential and Aquaculture Wastewater Treatment Ability of Penaeidins 3a Transgenic Duckweed.PMC1014458820JCQNJC00380No. 320716202020YJSS133Han BYang LHe YMa XWang WLi QLuo XWang YSun JRen QYu YfalseThe Antimicrobial Potential and Aquaculture Wastewater Treatment Ability of Penaeidins 3a Transgenic Duckweed.With the development of aquaculture, wastewater treatment and diseases have been paid more and more attention. The question of how to improve the immunity of aquatic species, as well as treat aquaculture wastewater, has become an urgent problem. In this study, duckweed with a high protein content (37.4%) (<i>Lemna turionifera</i> 5511) has been employed as a feedstock for aquatic wastewater treatment and the production of antimicrobial peptides. Penaeidins 3a (Pen3a), from <i>Litopenaeus vannamei</i>, were expressed under the control of CaMV-35S promoter in duckweed. Bacteriostatic testing using the Pen3a duckweed extract showed its antibacterial activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. Transcriptome analysis of wild type (WT) duckweed and Pen3a duckweed s2023-01-01T00:00:00Z2023 Apr2025-04-18T16:29:43.557Z2025-02-19T02:57:08.259ZS-EPMC101445883711193910.3390/plants12081715