biostudies-literatureLiu ZGuizhou University Natural Science Special (Special Post) Scientific Research Fund ProjectStrategic Pilot Science and Technology Project of Chinese Academy of SciencesThe National Key Research and Development Program of ChinaThe Natural Science Foundation of Guizhou ProvinceZhenghui LiuYu Liuizhou Provincial Department of Education Young Science and Technology Talent GrowthYongzhong Lu411https://www.ebi.ac.uk/biostudies/studies/S-EPMC10145569biostudies-literatureUnknown9(4)Cobweb disease is a fungal disease that can cause serious damage to edible mushrooms worldwide. To investigate cobweb disease in <i>Morchella sextelata</i> in Guizhou Province, China, we isolated and purified the pathogen responsible for the disease. Through morphological and molecular identification and pathogenicity testing on infected <i>M</i>. <i>sextelata</i>, we identified <i>Cladobotryum mycophilum</i> as the cause of cobweb disease in this region. This is the first known occurrence of this pathogen causing cobweb disease in <i>M</i>. <i>sextelata</i> anywhere in the world. We then obtained the genome of <i>C</i>. <i>mycophilum</i> BJWN07 using the HiFi sequencing platform, resulting in a high-quality genome assembly with a size of 38.56 Mb, 10 contigs, and a GC content of 47.84%. WJournal of fungi (Basel, Switzerland)Characterization and Genome Analysis of <i>Cladobotryum mycophilum</i>, the Causal Agent of Cobweb Disease of <i>Morchella sextelata</i> in China.PMC10145569XDA28080304grant No. Guida Tegang Hezi (2022) 17grant No. Qian Jiao Ji [2022] 119Qian Ke He Zhi Cheng (2021) Generally 200Guida Tegang Hezi (2022) 17grant No. 2021YFD1600401grant No. XDA280803042021YFD1600401Qian Jiao Ji [2022] 119grant No. Qian Ke He Zhi Cheng (2021) Generally 200Sossah FLLiu ZLi YLu YCong YKang JfalseCharacterization and Genome Analysis of <i>Cladobotryum mycophilum</i>, the Causal Agent of Cobweb Disease of <i>Morchella sextelata</i> in China.Cobweb disease is a fungal disease that can cause serious damage to edible mushrooms worldwide. To investigate cobweb disease in <i>Morchella sextelata</i> in Guizhou Province, China, we isolated and purified the pathogen responsible for the disease. Through morphological and molecular identification and pathogenicity testing on infected <i>M</i>. <i>sextelata</i>, we identified <i>Cladobotryum mycophilum</i> as the cause of cobweb disease in this region. This is the first known occurrence of this pathogen causing cobweb disease in <i>M</i>. <i>sextelata</i> anywhere in the world. We then obtained the genome of <i>C</i>. <i>mycophilum</i> BJWN07 using the HiFi sequencing platform, resulting in a high-quality genome assembly with a size of 38.56 Mb, 10 contigs, and a GC content of 47.84%. W2023-01-01T00:00:00Z2023 Mar2026-04-08T13:34:21.315Z2025-02-19T04:46:20.067ZS-EPMC101455693710886510.3390/jof9040411