biostudies-literaturePanteleev PVRussian Science Foundation438https://www.ebi.ac.uk/biostudies/studies/S-EPMC10145973biostudies-literatureUnknown13(4)Proline-rich antimicrobial peptides (PrAMPs) having a potent antimicrobial activity and a modest toxicity toward mammalian cells attract much attention as new templates for the development of antibiotic drugs. However, a comprehensive understanding of mechanisms of bacterial resistance development to PrAMPs is necessary before their clinical application. In this study, development of the resistance to the proline-rich bovine cathelicidin Bac7_1-22 derivative was characterized in the multidrug-resistant <i>Escherichia coli</i> clinical isolate causing the urinary tract infection. Three Bac7_1-22-resistant strains with ≥16-fold increase in minimal inhibitory concentrations (MICs) were selected by serially passaging after four-week experimental evolution. It was shown thatMembranesGenomic Insights into Bacterial Resistance to Proline-Rich Antimicrobial Peptide Bac7.PMC1014597321-74-00100Ovchinnikova TVKruglikov RNBolosov IASafronova VNPanteleev PVfalseGenomic Insights into Bacterial Resistance to Proline-Rich Antimicrobial Peptide Bac7.Proline-rich antimicrobial peptides (PrAMPs) having a potent antimicrobial activity and a modest toxicity toward mammalian cells attract much attention as new templates for the development of antibiotic drugs. However, a comprehensive understanding of mechanisms of bacterial resistance development to PrAMPs is necessary before their clinical application. In this study, development of the resistance to the proline-rich bovine cathelicidin Bac7_1-22 derivative was characterized in the multidrug-resistant <i>Escherichia coli</i> clinical isolate causing the urinary tract infection. Three Bac7_1-22-resistant strains with ≥16-fold increase in minimal inhibitory concentrations (MICs) were selected by serially passaging after four-week experimental evolution. It was shown that2023-01-01T00:00:00Z2023 Apr2025-04-21T17:26:04.266Z2025-04-05T16:40:46.737ZS-EPMC101459733710386510.3390/membranes13040438