biostudies-literatureLim HKorean Health Industry Development InstituteNational Research Foundation of Korea712https://www.ebi.ac.uk/biostudies/studies/S-EPMC10146744biostudies-literatureUnknown14(4)An early and accurate diagnosis of Candida albicans is critical for the rapid antifungal treatment of candidemia, a mortal bloodstream infection. This study demonstrates viscoelastic microfluidic techniques for continuous separation, concentration, and subsequent washing of Candida cells in the blood. The total sample preparation system contains two-step microfluidic devices: a closed-loop separation and concentration device and a co-flow cell-washing device. To determine the flow conditions of the closed-loop device, such as the flow rate factor, a mixture of 4 and 13 μm particles was used. Candida cells were successfully separated from the white blood cells (WBCs) and concentrated by 74.6-fold in the sample reservoir of the closed-loop system at 800 μL/min with a flow rate factor of 3.3.MicromachinesSeparation and Washing of Candida Cells from White Blood Cells Using Viscoelastic Microfluidics.PMC10146744HR20C00212020R1A2C1014460Lee CKim JYHan BJLim HChoo SLim CSNam JfalseSeparation and Washing of Candida Cells from White Blood Cells Using Viscoelastic Microfluidics.An early and accurate diagnosis of Candida albicans is critical for the rapid antifungal treatment of candidemia, a mortal bloodstream infection. This study demonstrates viscoelastic microfluidic techniques for continuous separation, concentration, and subsequent washing of Candida cells in the blood. The total sample preparation system contains two-step microfluidic devices: a closed-loop separation and concentration device and a co-flow cell-washing device. To determine the flow conditions of the closed-loop device, such as the flow rate factor, a mixture of 4 and 13 μm particles was used. Candida cells were successfully separated from the white blood cells (WBCs) and concentrated by 74.6-fold in the sample reservoir of the closed-loop system at 800 μL/min with a flow rate factor of 3.3.2023-01-01T00:00:00Z2023 Mar2025-07-09T03:04:24.041Z2025-04-07T03:52:40.494ZS-EPMC101467443742094710.3390/mi14040712