{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gong D"],"funding":["Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences","National Natural Science Foundation of China","Kunming Institute of Zoology, Chinese Academy of Sciences"],"pagination":["952-968"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10147553"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(4)"],"pubmed_abstract":["Embryonic stem cells (ESCs) are superior to differentiated cells to maintain genome stability, but the underlying mechanisms remain largely elusive. R-loops are constantly formed during transcription and are inducers of DNA damage if not resolved. Here we report that mouse ESCs (mESCs) can efficiently prevent unscheduled R-loop formation, and a long noncoding RNA Lnc530 plays regulatory role. Lnc530 is expressed in mESCs and localizes on R-loops. Depletion of Lnc530 in mESCs causes R-loop accumulation and DNA damage, whereas forced expression of Lnc530 in differentiated cells suppresses the R-loop formation. Mechanistically, Lnc530 associates with DDX5 and TDP-43 in an inter-dependent manner on R-loops. Formation of Lnc530-DDX5-TDP-43 complex substantially increases the local protein level"],"journal":["Stem cell reports"],"pubmed_title":["Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells."],"pmcid":["PMC10147553"],"funding_grant_id":["31930027"],"pubmed_authors":["Zhang W","Gong D","Gao J","Zheng P","Zhou H","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells.","description":"Embryonic stem cells (ESCs) are superior to differentiated cells to maintain genome stability, but the underlying mechanisms remain largely elusive. R-loops are constantly formed during transcription and are inducers of DNA damage if not resolved. Here we report that mouse ESCs (mESCs) can efficiently prevent unscheduled R-loop formation, and a long noncoding RNA Lnc530 plays regulatory role. Lnc530 is expressed in mESCs and localizes on R-loops. Depletion of Lnc530 in mESCs causes R-loop accumulation and DNA damage, whereas forced expression of Lnc530 in differentiated cells suppresses the R-loop formation. Mechanistically, Lnc530 associates with DDX5 and TDP-43 in an inter-dependent manner on R-loops. Formation of Lnc530-DDX5-TDP-43 complex substantially increases the local protein level","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2025-04-18T14:17:54.485Z","creation":"2025-04-07T00:24:00.664Z"},"accession":"S-EPMC10147553","cross_references":{"pubmed":["36931280"],"doi":["10.1016/j.stemcr.2023.02.003"]}}