{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(4)"],"submitter":["Feser R"],"pubmed_abstract":["MicroRNA (miRNA/miR) 526 b- and miR655-overexpressed tumor cell-free secretions regulate the breast cancer tumor microenvironment (TME) by promoting tumor-associated angiogenesis, oxidative stress, and hypoxic responses. Additionally, premature miRNA (pri-miR526b and pri-miR655) are established breast cancer blood biomarkers. However, the mechanisms of how these miRNAs regulate the TME has yet to be investigated. Mass spectrometry analysis of miRNA-overexpressed cell lines MCF7-miR526b, MCF7-miR655, and miRNA-low MCF7-Mock cell-free secretomes identified 34 differentially expressed proteins coded by eight genes. In both miRNA-high cell secretomes, four markers are upregulated: <i>YWHAB</i>, <i>SFN</i>, <i>TXNDC12</i>, and <i>MYL6B,</i> and four are downregulated: <i>PEA15</i>, <i>PRDX4</i>"],"journal":["Heliyon"],"pagination":["e15421"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10148110"],"repository":["biostudies-literature"],"pubmed_title":["Breast cancer cell secretome analysis to decipher miRNA regulating the tumor microenvironment and discover potential biomarkers."],"pmcid":["PMC10148110"],"pubmed_authors":["Feser R","Nault B","Chen VC","Opperman RM","Majumder M","Maiti S"],"additional_accession":[]},"is_claimable":false,"name":"Breast cancer cell secretome analysis to decipher miRNA regulating the tumor microenvironment and discover potential biomarkers.","description":"MicroRNA (miRNA/miR) 526 b- and miR655-overexpressed tumor cell-free secretions regulate the breast cancer tumor microenvironment (TME) by promoting tumor-associated angiogenesis, oxidative stress, and hypoxic responses. Additionally, premature miRNA (pri-miR526b and pri-miR655) are established breast cancer blood biomarkers. However, the mechanisms of how these miRNAs regulate the TME has yet to be investigated. Mass spectrometry analysis of miRNA-overexpressed cell lines MCF7-miR526b, MCF7-miR655, and miRNA-low MCF7-Mock cell-free secretomes identified 34 differentially expressed proteins coded by eight genes. In both miRNA-high cell secretomes, four markers are upregulated: <i>YWHAB</i>, <i>SFN</i>, <i>TXNDC12</i>, and <i>MYL6B,</i> and four are downregulated: <i>PEA15</i>, <i>PRDX4</i>","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2026-04-08T14:18:00.832Z","creation":"2025-04-04T14:18:31.102Z"},"accession":"S-EPMC10148110","cross_references":{"pubmed":["37128318"],"doi":["10.1016/j.heliyon.2023.e15421"]}}